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Bis-SNP Combined DNA methylation and SNP calling for Bisulfite-seq data.docVIP

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Bis-SNP Combined DNA methylation and SNP calling for Bisulfite-seq data.doc

    Bis-SNP Combined DNA methylation and SNP calling for Bisulfite-seq data

    Liuetal.GenomeBiology2012,13:R61 /2012/13/7/R61 METHOD OpenAccess Bis-SNP:CombinedDNAmethylationandSNP callingforBisulfite-seqdata YapingLiu1,2,KimberlyDSiegmund3,PeterWLaird1andBenjaminPBerman1,3* Abstract BisulfitetreatmentofDNAfollowedbyhigh-throughputsequencing(Bisulfite-seq)isanimportantmethodfor studyingDNAmethylationandepigeneticgeneregulation,yetcurrentsoftwaretoolsdonotadequatelyaddress singlenucleotidepolymorphisms(SNPs).IdentifyingSNPsisimportantforaccuratequantificationofmethylation levelsandforidentificationofallele-specificepigeneticeventssuchasimprinting.Wehavedevelopedamodel-

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