Characterization of β-N-acetylhexosaminidase (LeHex20A), a member of glycoside hydrolase family 20, from Lentinula edodes shiitake mushroom).docVIP

Konnoetal.AMBExpress2012,2:29 /content/2/1/29 ORIGINAL ARTICLE OpenAccess Characterizationofβ-N-acetylhexosaminidase (LeHex20A),amemberofglycosidehydrolase family20,fromLentinulaedodes(shiitake mushroom) NaotakeKonno*,HideyukiTakahashi,MasahiroNakajima,TakumiTakedaandYuichiSakamoto Abstract Wepurifiedandclonedaβ-N-acetylhexosaminidase,LeHex20A,withamolecularmassof79kDafromthefruiting bodyofLentinulaedodes(shiitakemushroom).Thegenelehex20agenehad1,659nucleotides,encoding553amino acidresidues.SequenceanalysisindicatedthatLeHex20Abelongstoglycosidehydrolase(GH)family20,and homologuesoflehex20a