滇桂艾纳香多糖BRP的结构解析.pdfVIP

2011 年第69 卷 化 学 学 报 Vol. 69, 2011 第9 期, 1101～1106 ACTA CHIMICA SINICA No. 9, 1101～1106 ·研究论文· 滇桂艾纳香多糖 BRP 的结构解析 许子竞 林翠梧* (广西大学化学化工学院 南宁 530004) 摘要 以滇桂艾纳香为原料, 经热水抽提、Sevag 法除蛋白、醇沉、DEAE-纤维素柱层析、Sephadex G-200、Sepharose 4 6 F.F.凝胶色谱纯化得到纯品BRP; 经HPGPC 检测分析表明, BRP 为均一多糖, 分子量为3.3 ×10 Da; 用UV 、IR 、 1 13 HPLC 、HPGPC 、GC-MS、甲基化、NMR ( H NMR 、 C NMR、HMQC 、HMBC)等方法对BRP 结构进行表征, 结果 表明, BRP 仅由呋喃果糖残基组成, 以→2)Fruf (1→ (或→1)Fruf (→2)方式链接, 推导其结构式为: β-D-Fruf -{2[→1)- β-D-Fruf (2→]n-1}-β-D-Fruf . 关键词 滇桂艾纳香多糖; 纯化; 核磁共振; 甲基化; 结构表征 Structure Identification of Polysaccharide BRP from Blumea Riparia Xu, Zijing Lin, Cuiwu* (College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004) Abstract A polysaccharide (BRP) was obtained from Blumea riparia, by hot water extraction, deproteination using Sevag method, precipitation with ethanol, and fractionation through DEAE-cellulose column, Sephadex G-200 and Sepharose 6 F.F. Gel filtration chromatography. Based on UV and IR spectrum, HPLC, HPGPC 1 13 and GC-MS chromatography, NMR ( H NMR, C NMR, HMQC, HMBC) technology and methylation reac- tion, the structure of BRP was identified. The results showed that BRP was hightly purified with average mo- 4 lecular weight of 3.3 ×10 Da determinted by HPGPC. And it was com