Eliminating the Interference of Oxygen for Sensing Hydrogen Peroxide with the Polyaniline Modified Electrode.docVIP

Sensors 2008, 8, 8237-8247; DOI: 10.3390/s8128237 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Eliminating the Interference of Oxygen for Sensing Hydrogen Peroxide with the Polyaniline Modified Electrode Yesong Gu* and Chien-Chung Chen Department of Chemical and Materials Engineering, Tunghai University, Taichung 40704, Taiwan * Author to whom correspondence should be addressed; E-mail: yegu@.tw (Y. G.); Tel.: +886 4 Fax: +886 4 2359009 Received: 19 October 2008; in revised form: 24 November 2008 / Accepted: 10 December 2008 / Published: 12 December 2008 Abstract: Polyaniline (PANI) has been shown to possess excellent catalytic activity toward oxygen reduction, however, this molecule may interfere with the electrochemical measurement of other targets when using a polyaniline modified platinum (PANI/Pt) electrode. In this study, we have demonstrated the considerable effects of dissolved oxygen on the sensing of hydrogen peroxide with the PANI/Pt electrode. Accordingly, we proposed a strategy to eliminate the influence of dissolved oxygen with oxygen scavengers. Our results indicated that as an oxygen scavenger sodium thiosulfate was very effective in the removal of dissolved oxygen from the sample solution, and had negligible effect on the quantification of hydrogen peroxide when its applied concentration was below 1 mM. Keywords: Amperometric electrode; polyaniline; hydrogen peroxide; oxygen scavenger 1. Introduction Polyaniline (PANI) has received considerable attention in biosensor fabrication because of its comparative conductivity, unique optical quality, and thermal stability [1-6]. The synthetic porous PANI matrix provides a suitable microenvironment for the immobilization of biocomponents, including enzymes, DNA, antibodies, and cells [7-14]. PANI-based biosensors have been successfully  Sensors 2008, 8 8238 applied in many fields, in particular, monitoring