Elicitor-induced transcription factors for metabolic reprogramming of secondary metabolism in Medicago truncatula.docVIP
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Elicitor-induced transcription factors for metabolic reprogramming of secondary metabolism in Medicago truncatula
BMC Plant Biology
BioMedCentral
Research article
Open Access
Elicitor-induced transcription factors for metabolic reprogramming
of secondary metabolism in Medicago truncatula
Marina A Naoumkina, XianZhi He and Richard A Dixon*
Address: Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA
Email: Marina A Naoumkina - manaoumkina@; XianZhi He - xianzhihe@; Richard A Dixon* - radixon@
* Corresponding author
Published: 22 December 2008
Received: 31 August 2008
Accepted: 22 December 2008
BMC Plant Biology 2008, 8:132
doi:10.1186/1471-2229-8-132
This article is available from: /1471-2229/8/132
? 2008 Naoumkina et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: Exposure of Medicago truncatula cell suspension cultures to pathogen or wound
signals leads to accumulation of various classes of flavonoid and/or triterpene defense molecules,
orchestrated via a complex signalling network in which transcription factors (TFs) are essential
components.
Results: In this study, we analyzed TFs responding to yeast elicitor (YE) or methyl jasmonate (MJ).
From 502 differentially expressed TFs, WRKY and AP2/EREBP gene families were over-represented
among YE-induced genes whereas Basic Helix-Loop-Helix (bHLH) family members were more over-
represented among the MJ-induced genes. Jasmonate ZIM-domain (JAZ) transcriptional regulators
were highly induced by MJ treatment. To investigate potential involvement of WRKY TFs in
signalling, we expressed four Medicago WRKY genes in tobacco. Levels of soluble and wall bound
phenolic compounds and lignin were increased in all cases. WRKY W109669 also induced tobacco
endo-1,3-β-glucanase (NtPR2)
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