Enhancement of Diepoxin ζ Production by Yeast Extract and Its Fractions in Liquid Culture of Berkleasmium-Like Endophytic Fungus Dzf12 from Dioscorea zingiberensis.docVIP

Molecules 2011, 16, 847-856; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Enhancement of Diepoxin ζ Production by Yeast Extract and Its Fractions in Liquid Culture of Berkleasmium-Like Endophytic Fungus Dzf12 from Dioscorea zingiberensis Jianglin Zhao, Bingbing Zheng, Yan Li, Tijiang Shan, Yan Mou, Shqiong Lu, Peiqin Li and Ligang Zhou * College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China * Author to whom correspondence should be addressed; E-Mail: lgzhou@; Tel.: +86 10 Received: 14 December 2010; in revised form: 7 January 2011 / Accepted: 18 January 2011 / Published: 19 January 2011 Abstract: This study was to examine the effects of yeast extract (YE) and its fractions (YE1 and YE2) on the growth and diepoxin ζ (a spirobisnaphthalene with a diversity of bioactivities) production in liquid culture of Berkleasmium-like endophytic fungus Dzf12 from Dioscorea zingiberensis. When YE was applied to the liquid medium at 10 g/L on day 3 of culture, the diepoxin ζ production was most effectively enhanced 3.2-fold (378.70 mg/L versus 120.09 mg/L in control) after another 10 days culture. Feeding with 15 g/L of YE on day 9, the mycelia biomass reached 16.44 g/L, about 2.3-fold in comparison with the control (7.15 g/L). The polysaccharide fraction (YE1) was mainly responsible for stimulating diepoxin ζ accumulation, and the non-polysaccharide fraction (YE2) was mainly responsible for promoting mycelia growth. The results showed that the diepoxin ζ production in liquid culture of endophyte Dzf12 could be effectively enhanced by YE and its fractions. Keywords: diepoxin ζ; Berkleasmium-like endophytic fungus Dzf12; Dioscorea zingiberensis; yeast extract (YE); polysaccharide fraction; non-polysaccharide fraction; enhancement  Molecules 2011, 16 848 1. Introduction Plant endoph