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Enrichment of sequencing targets from the human genome by solution hybridization.doc

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Enrichment of sequencing targets from the human genome by solution hybridization

Open Access M d 2009TewheyetVoal.lumeetho10, Issue 10, Article R116 Enrichment of sequencing targets from the human genome by solution hybridization Ryan Tewhey *?, Masakazu Nakano¤*§, Xiaoyun Wang*§, Carlos Pabón- ¤ Pe?a?, Barbara Novak?, Angelica Giuffre?, Eric Lin?, Scott Happe?, Doug N Roberts?, Emily M LeProust?, Eric J Topol*, Olivier Harismendy*§ and Kelly A Frazer*§ Addresses: *Scripps Genomic Medicine, Scripps Translational Science Institute, The Scripps Research Institute, 3344 N. Torrey Pines Court, La Jolla, CA 92037, USA. ?Division of Biological Sciences, University of California San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA. ?Agilent Technologies, Inc., 5301 Stevens Creek Blvd., Santa Clara, CA 95051, USA. §Current address: Moores UCSD Cancer Center, 3855 Health Sciences Drive 0901, La Jolla, CA 92093-0901, USA. ¤ These authors contributed equally to this work. Correspondence: Olivier Harismendy. Email: oharismendy@. Kelly A Frazer. Email: kafrazer@ Published: 16 October 2009 Received: 17 June 2009 Revised: 5 September 2009 Accepted: 16 October 2009 Genome Biology 2009, 10:R116 (doi:10.1186/gb-2009-10-10-r116) The electronic version of this article is the complete one and can be found online at /2009/10/10/R116 ? 2009 Tewhey; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium provided the original work is properly cited. SelectiveotpideApmroebexonthesodinfcaptureorsoltargetution hsequenceas excelleenrichmentnt sensitivitfromy andtheacchumanuracy fogenomer callingisSdescribed.NPs/p This hybridization-based approach using oligonucle- Abstract To exploit fully the potential of current sequencing technologies for population-based studies, one must enrich for loci from the human genome. Here we evaluate the hybridization-based approach by using oligo