Evaluation of oxidative stress in Insulin Dependent Diabetes Mellitus (IDDM) patients.docVIP

Diagnostic Pathology BioMedCentral Research Open Access Evaluation of oxidative stress in Insulin Dependent Diabetes Mellitus (IDDM) patients Vadde Ramakrishna*1,2 and Rama Jailkhani1 Address: 1Department of Biochemistry, Shri B.M. Patil Medical College, Bijapur 586 103, India and 2Department of Biotechnology, Sri Krishnadevaraya University, Anantapur 515 003, India Email: Vadde Ramakrishna* - vrkrishna70@yahoo.co.in; Rama Jailkhani - ramajailkhani@ * Corresponding author Published: 1 July 2007 Received: 1 April 2007 Accepted: 1 July 2007 Diagnostic Pathology 2007, 2:22 doi:10.1186/1746-1596-2-22 This article is available from: /content/2/1/22 ? 2007 Ramakrishna and Jailkhani; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Free radical mediated oxidative stress is mainly involved in the pathogenesis of diabetic complications. Proteins and lipids are among the prime targets for oxidative stress. In the present study, we evaluated the oxidative stress in chronic IDDM patients by estimating the lipid peroxidation, protein oxidation, and antioxidants status. Subjects and design: A total of 35 (15 IDDM + 20 normal healthy) children were examined in the study and estimated the lipid peroxidation, protein oxidation, and antioxidants – vitamin A (β- carotene, retinol), vitamin C, vitamin E and enzymatic antioxidants and nitric oxide. Results: A statistically significant higher values of protein carbonyl groups and MDA as lipid peroxides were observed in diabetic patients with slight reduction in the synthesis of nitric oxide. It is interesting to note that there was a decrease in the antioxidant levels with corresponding increased protein and lipid oxidation. On PA