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Evaluation of Petrifilm？ Select E. coli Count Plate medium to discriminate antimicrobial resistant Escherichia coli.doc

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Evaluation of Petrifilm？ Select E. coli Count Plate medium to discriminate antimicrobial resistant Escherichia coli

Acta Veterinaria Scandinavica BioMedCentral Research Open Access Evaluation of Petrifilm? Select E. coli Count Plate medium to discriminate antimicrobial resistant Escherichia coli Shuyu Wu*1,2, Eirini Chouliara1,2, Lars Bog? Jensen1 and Anders Dalsgaard2 Address: 1Research Group of Antimicrobial Resistance and Molecular Epidemiology, Department of Microbiology and Risk Assessment, National Food Institute, Technical University of Denmark, Bülowsvej 27, DK-1790 Copenhagen V, Denmark and 2Department of Veterinary Pathobiology, Faculty of Life Sciences, University of Copenhagen, Gr?nneg?rdsvej 15, DK-1870 Frederksberg C, Denmark Email: Shuyu Wu* - wshuy@food.dtu.dk; Eirini Chouliara - echoul@cc.uoi.gr; Lars Bog? Jensen - lboj@food.dtu.dk; Anders Dalsgaard - ad@life.ku.dk * Corresponding author Published: 25 September 2008 Received: 1 8 July 2008 Accepted: 25 September 2008 Acta Veterinaria Scandinavica 2008, 50:38 doi:1 0.1 1 86/1 751 -01 47-50-38 This article is available from: /content/50/1 /38 ? 2008 Wu et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Screening and enumeration of antimicrobial resistant Escherichia coli directly from samples is needed to identify emerging resistant clones and obtain quantitative data for risk assessment. Aim of this study was to evaluate the performance of 3M? Petrifilm? Select E. coli Count Plate (SEC plate) supplemented with antimicrobials to discriminate antimicrobial-resistant and non-resistant E. coli. Method: A range of E. coli isolates were tested by agar dilution method comparing the Minimal Inhibitory Concentration (MIC) for eight antimicrobials obtained by Mueller-Hinton II agar, MacConkey agar and SEC plates. Ka