Evaluation of protein pattern changes in roots and leaves of Zea mays plants in response to nitrate availability by two-dimensional gel electrophoresis analysis.docVIP

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Evaluation of protein pattern changes in roots and leaves of Zea mays plants in response to nitrate availability by two-dimensional gel electrophoresis analysis.doc

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Evaluation of protein pattern changes in roots and leaves of Zea mays plants in response to nitrate availability by two-dimensional gel electrophoresis analysis

BMC Plant Biology BioMedCentral Research article Open Access Evaluation of protein pattern changes in roots and leaves of Zea mays plants in response to nitrate availability by two-dimensional gel electrophoresis analysis Bhakti Prinsi1, Alfredo S Negri1, Paolo Pesaresi2, Maurizio Cocucci1 and Luca Espen*1 Address: 1Dipartimento di Produzione Vegetale, University of Milan, via Celoria 2, I-20133 Milano, Italy and 2Dipartimento di Produzione Vegetale, University of Milan c/o Fondazione Parco Tecnologico Padano, via Einstein – Località Cascina Codazza, I-26900 Lodi, Italy Email: Bhakti Prinsi - bhakti.prinsi@unimi.it; Alfredo S Negri - alfredo.negri@unimi.it; Paolo Pesaresi - paolo.pesaresi@unimi.it; Maurizio Cocucci - maurizio.cocucci@unimi.it; Luca Espen* - luca.espen@unimi.it * Corresponding author Published: 23 August 2009 Received: 2 April 2009 Accepted: 23 August 2009 BMC Plant Biology 2009, 9:113 doi:10.1186/1471-2229-9-113 This article is available from: /1471-2229/9/113 ? 2009 Prinsi et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Nitrogen nutrition is one of the major factors that limit growth and production of crop plants. It affects many processes, such as development, architecture, flowering, senescence and photosynthesis. Although the improvement in technologies for protein study and the widening of gene sequences have made possible the study of the plant proteomes, only limited information on proteome changes occurring in response to nitrogen amount are available up to now. In this work, two-dimensional gel electrophoresis (2-DE) has been used to investigate the protein changes induced by NO3- concentration in both roots and leaves of maize (Zea mays L.)