Fine mapping in tomato using microsynteny with the Arabidopsis genome the Diageotropica (Dgt) locus.docVIP

/2002/3/9/research/0049.1 Research Fine mapping in tomato using microsynteny with the Arabidopsis genome: the Diageotropica (Dgt) locus KwangChul Oh*, Kristine Hardeman*?, Maria G Ivanchenko*, Mary Ellard- Ivey*?, Andreas Nebenführ*§, TJ White* and Terri L Lomax* Addresses: *Department of Botany and Plant Pathology and the Center for Gene Research and Biotechnology, Oregon State University, Corvallis, OR 97331-2902, USA. ?Monsanto-Mystic Research, 62 Maritime Drive, Mystic, CT 06355, USA. ?Department of Biology, Pacific Lutheran University, Tacoma, WA 98447-0003, USA. §Department of Botany, University of Tennessee, Knoxville, TN 37996-1100, USA. Correspondence: Terri L Lomax: lomaxt@ Published: 28 August 2002 Received: 18 December 2001 Revised: 28 May 2002 Accepted: 21 June 2002 Genome Biology 2002, 3(9):research0049.1–0049.11 The electronic version of this article is the complete one and can be found online at /2002/3/9/research/0049 ? 2002 Oh et al., licensee BioMed Central Ltd (Print ISSN 1465-6906; Online ISSN 1465-6914) Abstract Background: The Arabidopsis thaliana genome sequence provides a catalog of reference genes applicable to comparative microsynteny analysis of other species, facilitating map-based cloning in economically important crops. We have applied such an analysis to the tomato expressed sequence tag (EST) database to expedite high-resolution mapping of the Diageotropica (Dgt) gene within the distal end of chromosome 1 in tomato (Lycopersicon esculentum). Results: A BLAST search of the Arabidopsis database with nucleotide sequences of markers that flank the tomato dgt locus revealed regions of microsynteny between the distal end of chromosome 1 in tomato, two regions of Arabidopsis chromosome 4, and one on chromosome 2. Tomato ESTs homeologous to Arabidopsis gene sequences within those regions were converted into co-dominant molecular markers via cleaved amplified polymorphi