梁玮论文.doc

本科毕业论文 被毛转拟蛋白 学 院：生命科学学院 专 业：生物科学 姓 名：梁玮 学 号：091114557 指导教师：孟凡华 职 称：讲 师 论文提交日期：二Ο一三年六月 摘 要 羊毛主要由蛋白质组成，是主要的纺织原料。羊毛的性能主要是由编码羊毛蛋白的基因决定的，所以采用生物工程技术引入优良基因结合动物克隆手段生产转基因克隆动物是改变羊毛品质的有效途径。蜘蛛丝的主要成分也是蛋白质，高强度、高弹性、高断裂功，有很高的利用价值。通过转基因技术使蜘蛛牵丝蛋白基因在山羊被毛中特异表达，将能有效改变羊毛的机械性能。本实验以转拟蜘蛛牵丝蛋白基因山羊为材料，通过提取胎盘，得基因组，再对其DNA、RNA水平PCR鉴定。再取转基因羊与自然羊不同部位羊毛进行羊毛拉力测试。结果显示拟蜘蛛牵丝蛋白基因成功转入山羊DNA中；并且在山羊RNA中成功表达，此外通过羊毛拉力应力测试仪测的结果显示克隆羊不同部位的羊毛比自然羊的拉伸度、强度都明显增高。 关键词：蜘蛛牵丝蛋白基因；克隆羊胎盘；羊毛拉力；转基因羊鉴定 Identification of clothing hair turning dragline silk protein gene of cloned sheep Abstract The wool is mainly composed of protein,and it is the major textile raw materials. Wool performance is mainly determined by the wool protein encoding gene, so the use of biological engineering technology into the excellent combination of animal cloning gene in transgenic animal production is an effective way to change the wool quality. Spider silks are also mainly constituted by proteins, with the excellent characteristics of high strength, high flexibility, high work of fracture, in which the performance of the dragline silk is the best. It can perhaps effectively change the mechanical properties of wool to express the Spider Dragline Silk Protein Gene specifically in the hair follicle of sheep through transgenic technology. In this experiment, goats as material,through extracting the placenta, getting a genome.Then make it the DNA, RNA level in PCR identification. Then extract wool of transgenic sheep and general sheep in different parts to tensile test. The results show that artificial spider dragline silk protein gene was transferred into the goat DNA;expressed successfully in the goat RNA. Bying wool pulling force measurement results show that the wool in different parts of the cloned sheep were significantly higher than natural sheep, tensile strength. Keywords: Spider Dragline Silk like protein gene; The cloned sheep placenta; wool tension; identific