大叶柴胡鉴别.doc

Report Project: Bupleurum longiradiatum（大叶柴胡） Customer name and address: YUAN Ye / NKI-TCM Project objective: Identification Bupleurum longiradiatum Date: 6th May 2012 Laboratory: NKI-TCM 1. TLC Test solution. Heat 0.5 g of the powdered drug in 30ml of methanol R under a reflux condenser for 10 min. Cool then filtered and evaporates the solvent to dryness. Take up the residue in 5 mL of the methanol. Reference solution. Dissolve 1 mg of Saikosaponin D R in 1 ml of methanol R. Plate: TLC silica gel plate R (2-10 μm). Mobile phase: ethyl acetate R, ethanol R, water R (8:2:1 V/V). Application: 10 μL as bands. Development: over a path of 8 cm. Drying: in air Detection: spray the warm plate with 2g/100ml p-dimethyl aminobenzaldehyde in 40% sulfuric acid and dry at 60℃ for 20min. Examine in ultraviolet light at 365 nm. Left: Bupleurum longiradiatum Right: radix bupleuri Detection under sunlight Left: Bupleurum longiradiatum Right: radix bupleuri Examine in ultraviolet light at 365 nm 2. Ultraviolet spectrophotometry Test solution. 0.15g of the powdered drug (355) in 50 ml of ethanol R sonicate at room time for 10 minutes, filter and dilute to 100.0ml with ethanol R. Wavelength range: 200-350. Instrument Type: UV-2500PC series Result: Sample contains three characteristic absorption peaks: 336.8, 315.8, and 297.0. But radix bupleuri don not have them. Red: Sample ( Bupleurum longiradiatum) Green: radix bupleuri 3. Conclusion: The sample is radix of Bupleurum longiradiatum.