子宫内膜样腺癌及癌前病变组织中PTEN蛋白的表达.docVIP

子宫内膜样腺癌及癌前病变组织中PTEN蛋白的表达 【摘要】 目的 探讨子宫内膜样腺癌及癌前病变组织中PTEN蛋白表达的关系。 方法 采用免疫组化SP法检测正常子宫内膜、内膜增生、不典型性增生和子宫内膜样腺癌组织中PTEN蛋白的表达。结果 不典型性增生和子宫内膜样腺癌组中PTEN蛋白阳性表达率分别为50.00%、45.46%，两组中PTEN阳性表达均低于正常子宫内膜和子宫内膜增生组（P0.01）。癌组中PTEN阳性表达的缺失与组织学分级（P0.01）、肌层浸润深度或伴有转移（P0.05）及临床分期（P0.01）有关，肿瘤的复发与PTEN的表达缺失有关（P0.05）。结论 PTEN表达缺失涉及了子宫内膜样腺癌的发生、 发展 过程，并与肿瘤的复发密切有关，检测PTEN可作为子宫内膜样腺癌的早期诊断、判断肿瘤生物学行为的一个指标。 【关键词】 癌 子宫内膜样；免疫组织化学 PTEN Abstract: Objective To investigate the expressions of PTEN protEin in endometrial adenocarcinoma and lesion tissues of precancers. Methods An immunohistochemistry SP method was used to detect the expressions of PTEN protein in normal endometrium tissues, endometrial hyperplasia tissues, atypical endometrial hyperplasia tissues and endometrial adenocarcinoma tissues. Results The positive expression of PTEN protein in atypical hyperplasia and endometrial adenocarcinoma groups were 50.00% and 45.46%, respectively. The positive expressions of PTEN in the two groups were lower than those in the normal endometrium and endometrial hyperplasia groups (P0.01). In adenocarcinoma group, the loss of PTEN expression was associated with the histological classification (P0.01), depth of skin invasiveness or metastasis (P0.05) and clinical stage (P0.01). The relapse of tumors is related to the loss of PTEN expression (P0.05). Conclusion The loss of PTEN expression is involved in the genesis and development of endometrial adenocarcinoma, and is associated positively with the relapse of tumors. PTEN can be used as a marker for early diagnosis of endometrial adenocarcinoma and prediction for tumor biological behavior. 　　Key words: carcinoma, endometrioid; immunohistochemistry; PTEN 肿瘤的本质在于细胞周期调节机制失控，导致细胞异常增殖所致，PTEN作为编码具有磷酸酶活性产物的抑癌基因对细胞周期G1期的负调控起着关键的作用。我们采用免疫组化技术检测子宫内膜样腺癌及癌前病变组织中PTEN蛋白，旨在探讨PTEN表达异常在子宫内膜样腺癌发生、发展中的作用及其与临床病理特征之间的相互关系，为临床早期诊断、 治疗 和评价预后提供依据。 　　 1 材料与方法 　　1.1 病例来源 　　选自广西柳州市妇幼保健院病理科1996年6月～2002年6月的子宫内膜病变标本，全部病例均经病理诊断证实。病理诊断、分类参照WHO的分类标准进行［1］。其中子宫内膜增生组30例（18例为单纯性，12例为复杂性），年