高家敏,曹进,丁宏-食品安全质量检测学报.doc

高效液相色谱法测定保健食品胶囊剂硬胶囊壳中合成着色剂 高家敏曹进丁宏 (中国食品药品检定研究院北京100050) 摘 要: 目的 建立高效液相色谱法(high performance liquid chromatography, HPLC)测定保健食品胶囊剂硬胶囊壳中合成着色剂的分析方法。方法硬胶囊壳用热水溶解, 加入聚酰胺粉吸附着色剂, 用甲醇-甲酸(V:V, 6:4)洗脱天然着色剂, 再用乙醇-氨水-水(V:V:V, 7:2:1)解吸合成着色剂, 随后使用HPLC上机检测检测新红、柠檬黄、苋菜红、靛蓝、胭脂红、日落黄、亮蓝、赤藓红8种合成着色剂, 外标法定量。结果8种合成着色剂均可得到良好的分离, 在5～200 μg/mL范围内线性良好,相关系数均大于0.999,平均回收率(n=9)均在90%以上。应用该方法对10批保健食品胶囊剂彩色硬胶囊壳中的合成着色剂进行测定, 均检出国家标准GB 2760-2014《食品安全国家标准 食品添加剂使用标准》允许使用的合成着色剂。结论该方法准确、可靠, 适用于保健食品胶囊剂硬胶囊壳中合成着色剂的测定。 关键词保健食品; 硬胶囊壳; 合成着色剂; 高效液相色谱法 synthetic colorants in health food capsule shells by high performance liquid chromatography GAO Jia-Min, CAO Jin*, DING Hong* (National Institutes for Food and Drug Control, Beijing 100050, China) ABSTRACT: Objective To establish a method for determination of synthetic colorants in health food capsule shells by high performance liquid chromatography (HPLC). Methods The capsule shells were dissolved in hot water and adsorbed by polyamide, then the natural colorants in samples were eluted by methanol- formic acid(V:V, 6:4)and then the synthetic colorants were desorbed by ethanol-ammonium hydroxide-water (V:V:V, 7:2:1). Eight kinds of synthetic colorants in samples were detected by HPLC. The quantification was performed by the external standard method. Results The chromatographic peaks of eight synthetic colorants were well separated. The linear ranges of all of them were 5~200 μg/mL, and the average recoveries (n=9) were all above 90%. The contents of synthetic colorants in 10 batches of health food capsule shells were determined by this method and the synthetic colorants detected in each batch of health food capsule shells. The detected synthetic colorants were allowed according to GB 2760-2014 National food safety standard Standard for uses of food additives. Conclusion This method is accurate and reliable, which can be applied to detect synthetic colorants in health food capsule shells. KEY WORDS: health food; capsule shell; synthetic colorant; high performance