瑞舒伐他汀对冠心病患者单核巨噬细胞ABCA1及SREBP2表达的影响.docVIP

瑞舒伐他汀对冠心病患者单核巨噬细胞ABCA1及SREBP2表达的影响 作者：张培东 刘映峰 郭阳 缪绯 【摘要】 目的 探讨瑞舒伐他汀对冠心病（CHD）患者单核巨噬细胞ATP结合盒转运蛋白A1(ABCA1)mRNA和固醇调节元件结合蛋白2（SREBP2）mRNA表达的影响。方法 体外分离并培养CHD患者单核细胞，经佛波酯（PMA）诱导48 h成为巨噬细胞后，分别给予0、0.1、1、5、10 μmol/L瑞舒伐他汀干预;提取细胞总RNA，用实时荧光相对定量PCR(实时RTPCR)检测ABCA1和SREBP2 mRNA的表达。结果 不同浓度瑞舒伐他汀干预组单核巨噬细胞ABCA1 mRNA表达水平存在差异（P＜0.05），给予0.1、1、5、10 μmol/L瑞舒伐他汀干预组的ABCA1 mRNA表达水平与空白对照组比较均有降低（P＜0.05）。不同浓度瑞舒伐他汀干预组SREBP2 mRNA表达量也存在差异（P＜0.05）,并且与对照组比较均有明显升高（P＜0.05）。结论 在CHD外周血来源单核巨噬细胞干预模型中，瑞舒伐他汀能下调胆固醇逆转运相关基因ABCA1 mRNA的表达，并且上调细胞内胆固醇合成相关基因SREBP2 mRNA 的表达。 【关键词】 瑞舒伐他汀；ATP结合盒转运蛋白A1；固醇调节元件结合蛋白2 【Abstract】 Objective To investigate the effect of rosuvastatin on expressions of ATP binding cassette transporter A1 (ABCA1) and Sterol regulatory element binding protEin 2 (SREBP2) mRNA in monocytes and macrophages of coronary heart disease (CHD). Methods Monocytes were isolated by density gradient centrifugation with separating medium. Cultured monocytes were induced into macrophages by phorbol myristate acetate (PMA) for 48 h. Each group were incubated with 0, 0.1, 1, 5 and 10 μmol/L rosuvastatin respectively. Total RNA of these cells were abstracted and real time RTPCR were performed to inspect the expressions of ABCA1 and SREBP2 mRNA in the cells. Results Amplification products of ABCA1 and SREBP2 have excellent specificity. The groups of different concentrations of rosuvastatin had different expression of ABCA1 mRNA levels (P＜0.05) in monocyte macrophages. The expressions of ABCA1 mRNA in groups given rosuvastatin were lower than those of control group (P＜0.05). The groups of different concentrations of rosuvastatin also have different expression of SREBP2 mRNA levels (P＜0.05).The expressions of SREBP2 mRNA in groups given rosuvastatin were higher than those of control group (P＜0.05). Conclusions In the model of macrophage induced from monocytes of CHD patients, the expression of ABCA1 mRNA can be downregulated and SREBP2 mRNA can be upregulated by Rosuvastatin. 【Key words】 Rosuvastatin; ATP bi