结直肠癌IL-10和IL-12的检测及意义.docVIP

结直肠癌IL-10和IL-12的检测及意义 作者：刘新纯 姚联波 廖茂湖 张磊 【摘要】 目的 检测IL-10和IL-12在结直肠癌组织中含量及术前和术后3周血清浓度并探讨其意义。 方法 采用ELISA法检测了46例结直肠癌IL-10和IL-12在癌组织和癌周组织中含量及术前和术后3周血清浓度，与24例良性结肠息肉患者资料为对照。结果 结直肠癌组织IL-10含量高于癌周组织，而癌组织IL-12含量低于癌周组织；、期患者癌组织和癌周组织中IL-10含量高于、期， 而、期癌组织和癌周组织中IL-12含量高于、。术前和术后1周血清IL-10升高而IL-12降低，术后3周血清IL-10和IL-12浓度接近对照组。癌组IL-10和IL-12呈明显负相关。结论 结直肠癌细胞可分泌IL-10抑制IL-12的产生，进而营造免疫抑制微环境，动态检测IL-10和IL-12可反映结直肠癌病期、判断疗效和估计预后。 【关键词】 结直肠癌；IL-10；IL-12 The examination and clinical significance of IL-10 and IL-12 in colorectal carcinoma ［Abstract］ Objective To investigate the clinical significance of interleukin-10 (IL-10) and interleukin-12 (IL-12) in colorectal carcinoma. Methods The content of IL-10 and IL-12 were examined in colorectal carcinoma and its adjacent tissues by ELISA assay, and so did the concentration in the serum before operation， 1 and 3 weeks after operation in 46 cases of colorectal carcinoma and 24 cases of benign colonic polyp chosen as control. Results The content of IL-10 in carcinoma tissues was higher significantly than that in adjacent tissues, and contra versus about IL-12. The contents of IL-10 were higher in Ⅲ and Ⅳ stages than that of Ⅰ and Ⅱstages, and contra versus about IL-12. The concentrations of serum IL-10 decreased before and one week after operation, and contra versus about IL-12. But 3 weeks after operation, the level of IL-10 and IL-12 reached proximally to the level of the control group. There was negative correlation between IL-10 and IL-12 in tumor tissues and serum. Conclusion In patients with colorectal carcinoma, carcinoma cell may secrete IL-10 to suppress IL-12, which therefore creates microenvironment of immune suppression. of IL-10 and IL-12 can reflect colorectal carcinoma stage, judge curative effect and estimate prognosis. ［Key words］ Colorectal carcinoma;Interleukin-10;Interleukin-12 肿瘤细胞能分泌抑制性细胞因子，抑制患者免疫效应细胞的粘附能力与杀伤能力，或者释放可溶性抗原分子封阻抗体介导的细胞毒效应（antibody dependent cell mediated cytotoxicity, ADCC） ［1］；而且肿瘤细胞分泌的抑制性细胞因子，可以以旁分泌的形式，弥散在肿瘤组