联合检测大肠癌组织中PTEN与CyclinD1的表达及DNA含量的临床意义.docVIP

联合检测大肠癌组织中PTEN与CyclinD1的表达及DNA含量的临床意义 【摘要】 目的：探讨大肠癌组织中PTEN和CyclinD1的表达及与DNA含量联合检测的关系及意义。方法：应用免疫组织化学SP法检测58例大肠癌及14例癌旁正常大肠黏膜组织中PTEN和CyclinD1蛋白表达;应用流式细胞术检测以上组织中PTEN和CyclinD1的DNA含量;分析它们之间及与肿瘤分期、分级的关系。结果：PTEN蛋白在大肠癌组织中的表达(65.52%)显著低于癌旁正常组织(100%),CyclinD1蛋白在大肠癌组织中的表达(60.34%)显著高于癌旁正常组织(1.72%),两种蛋白在大肠癌组织中的表达呈负相关(r =-0.71);大肠癌组织的异倍体率(68.97%)显著高于癌旁正常组织(0);PTEN阳性组的DNA指数(DNA index，DI)、S期细胞比率(S-phase fraction ，SPF)均低于阴性组(P0.05)，CyclinD1阳性组的DI及SPF均高于阴性组(P0.05);两者的表达与肿瘤病理分级、Dukes分期及淋巴结转移相关;淋巴结转移患者的异倍体率及SPF均高于无淋巴转移患者(P0.05)。结论：PTEN和CyclinD1基因的异常改变可能参与大肠黏膜细胞的恶性转化过程，两者的变化存在相关性;DNA含量的变化可能与淋巴结转移有关。联合检测PTEN、CyclinD1蛋白及DNA含量可作为评估大肠癌病理生物学行为和预后的重要指标。 【关键词】 大肠肿瘤·癌基因·DNA，肿瘤 　　【ABSTRACT】 Objective: To investigate the expression of PTEN、CyclinD1 and DNA content in the tissue of colorectal carcinomas, and to discuss thEir relationships. Methods: The expression of PTEN and CyclinD1 in 58 cases of human colorectal carcinomas and 14 case of tumor-side normal tissue were detected by immunohistochemical technique; DNA content was measured by flow cytometry. Results: The positive expressive rate of PTEN in the colorectal carcinoma was 65.52%，which was lower than 93.10% of the tumor-side normal tissue, (P0.01). The positive expressive rate of CyclinD1 in the colorectal carcinoma was 60.34%,which was higher than 1.72% of the normal tissue. The expression of PTEN and CyclinD1 showed a negative correlation with each other (r =--0.71). The DNA index (DI) and sphase fraction (SPF) in the positive group of PTEN were lower than those in the negative group，and the DI in the positive group of CyclinD1 was higher than this in the negative group (P0.05). The expression of PTEN and CyclinD1 in colorectal carcinoma were associated with degree of differentiation of carcinoma, Dukes stage and lymph node metastasis of the tumors;the aneuploid ratio and SPF in lymph node metastasis positive patients were higher than those in the negative patients (P0.05). Conclusion: The abnormal expression of PTEN and CyclinD1