应用电子捕获解质谱对蛋白质磷酸化位点的定位.pptVIP

Large Scale Localization of ProteinPhosphorylation by Use of Electron Capture Dissociation Mass Spectrometry;Steve M. M. Sweet?§, Christopher M. Bailey?§, Debbie L. Cunningham?§,John K. Heath?§, and Helen J. Cooper§?;Content;1. Introduction of background;2. Experiment procedures;3. Analysis of Results;FIG. 1. Overlap between DTAs leading to identifications by ECD and CID. All 4763 identifications (IDs) are from paired CID/ECD events .FIG. 2. Overlap between distinct phosphopeptides identified by ECD and CID. All 1220 identifications are from paired CID/ECDevents. ;FIG. 3. Binned distribution