基于3-周期性的基因预测.PDF

安徽农业大学学报, 2014, 41(3): 351-357 Journal of Anhui Agricultural University [DOI] 10.13610/ki.1672-352x032 网络出版时间：2014-4-23 15:08:24 [URL]/kcms/doi/10.13610/ki.1672-352x032.html 基于 3-周期性的基因预测 胡连果，李 强，张 瑾，张良云，杨 涛，骈 聪 (南京农业大学理学院，南京 210095) 摘 要：基因预测是DNA 序列分析的一项重要任务，真核生物基因外显子序列的识别是生物信息学中的难点， 本研究提出一种基于 3-周期性小鼠基因的预测方法。基于碱基幅角的功率谱，使小鼠外显子序列的频谱图具有更 显著的 3-周期性，且内含子的频谱图更加平稳，从而增大了序列中外显子与内含子的信噪比的区分度，使小鼠基 因序列的预测获得更好的效果，在平均值法阈值 R2 下预测准确率达 94.53%；在分布图法阈值 R1 下，准确率达 94.50% ，敏感性达99.45%。 关键词：功率谱；3-周期性；幅角；信噪比；阈值 中图分类号：Q811.4 文献标识码：A 文章编号：1672−352X (2014)03−0351−07 An approach to gene prediction based on 3-periodicity HU Lianguo, LI Qiang, ZHANG Jin, ZHANG Liangyun, YANG Tao, PIAN Cong (College of Science, Nanjing Agricultural University, Nanjing 210095) Abstract: Gene prediction is a significant task, in which the recognization of exon of eukaryotic gene is a great challenge. In this study, a gene prediction method which is based on 3-periodicity in gene sequences was performed. A more prominent peak value in exon and more steady values in intron were shaped in the power spectrum curves based on arguments of the four bases. The distinction degree of SNR of exons and introns was enlarged and a better effect in short gene prediction was presented. In the condition of threshold R2, the accuracy reached to 94.53%; under the threshold R1, the accuracy reached to 94.50%, and the sensibility reached to 99.45%. Key words: power spectrum; 3-periodicity; argument; SNR; threshold 自人类基因组计划的展开和各种生物体测序的 构因子等等；②基于基因序列碱基的 3-周期性，所 完成，生命科学研究进入了一个全新的阶段，对于 谓碱基的 3-周期性，即若对基因序列进行数值化映 基因组的碱基序列进行测定，破解遗传信息是一项