致羔羊脑炎粪肠球菌生物被膜形成能力及相关基因检测研究.pdfVIP

2017 44 3 679685 China A nim al H usbandry Vete r inary M e d ic ine doi cnki j XJ BF BF XJ BF D nm BF BF elEesp aceasaasaefaA ef g ef ahrceep XJ BF P h D nm P h BF h h h BF XJ BF BF S A Detection on Biofilm and Biofilmassociated Genes of Enterococcus f aecalis Inducing Lamb Encephalitis KONG Jing a GUO Qiang iang KONG Kew ei LI Jie Z HOU Xia QI Ya in y q y College of A nimal Science and Technology Shihez i Univ ersity Shihez i China Abstract T he purpose of this study w as to observe and analyze the ability of biofilm f ormation of the Ente rococc us aecalis E aecalis XJ and to detect if the E aecalis w hich could induce f f f lamb encephalitis carried the biofilm associated genes T he E aecal is XJ w as used as the sam f ple T he biofilm model w as established by the w ell microtiter plate and read by microplate reader in the D nm at the same time observed by the inverted microscope T he primers w ere de sig ned to ex amine if the strains of E aecalis carried the biofilm related genes and then the f amplified product s w ere sequenced and analyzed f or homology T he result s show ed that the a mount of BF produce