细胞计数(国外英文资料).docVIP

细胞计数 Steps: 1. 10ul cell suspension and 10ul tai hope orchid mixed with 1.5 ml centrifuge. Cover the cover glass, take 10ul mixed fluid from the blood ball counter top, observe in 10 times biologic microscope, living cell not dyeing, dead cells are dyed blue. 3. Count four large squares, respectively, to get the total number of cells, divided by four, multiplied by the dilution ratio for 2) (in this experiment, finally multiplied by 104, the per ml cells in suspension cell number. If the cell is on the line, only the line and the left line are counted. Note: 1. Cell count/ml = 4 large cells. The volume of each large lattice is equal to 0.1 cm x 0.1 cm x 0.01 cm = 10-4ml When counting board counts, the optimal cell concentration is 5 ~ 10 x 105 / ml, and the outside counting error is large. High concentration of cell suspension, which can be partially diluted or continuously diluted. Cell counting is a method used to count the number of cells in a cells suspension. Normally use the counting board (blood ball counting board). Can be used to separate (powder) cell culture inoculation before counting the number of cells in cell suspension preparation by, also can be used to culture cell number count. The dispersed cell suspension is required, regardless of the object of the count. The preparation of cell suspension For the cells in suspension, the following steps 2 (counting and counting) are performed directly. If the counting object is a cell that grows on the wall, the first thing you need to do is make a cell suspension. 1), end culture, suck the culture medium out, use PBS to wash the culture material once. 2), 1 ml to join in the culture bottle, 0.25% trypsin solution in 37 ℃ digestive 3 ~ 5 min. Constantly under the microscope. As the cells get round to the wall, the digestive juices are discarded. 3), add a certain amount of culture (if these cultured cells are no longer useful, add PBS), blow with a straw, and make the cells take off the wall and make a cell s