东北山樱根际细菌群落结构多样性分析.pdfVIP

吉林农业大学学报 2011，09 Journal of Jilin Agricultural University E-mail:jlndxb@ 东北山樱根际细菌群落结构多样性分析∗ 1 1** 2 1 1 1 秦嗣军 ，吕德国 ，李志霞 , 刘灵芝 ，刘国成 ，马怀宇 1.沈阳农业大学园艺学院，沈阳 110161; 2. 中国农业科学院果树研究所，兴城 125100 摘 要: 利用 PCR-DGGE 技术研究了野生与栽培两种生境下东北山樱的表层和根际土壤细菌群落结构的差 异，以及野生东北山樱根际与其周围优势植物根际细菌群落结构的区别。运用细菌的 16S rDNA 特异引物对， 对东北山樱和相邻其他优势植物根区土壤中提取的总 DNA 进行 PCR 扩增，通过 DGGE 技术对 PCR 产物进 行分析。试验结果表明：无论在栽培条件下还是在野生条件下，东北山樱的根际土壤细菌群落结构多样性 都高于表层土，且栽培条件下的多样性更高；野生东北山樱的根际土壤中形成特有的细菌群落，但其多样 性低于对照和相邻的优势植物。 关键词: 东北山樱；细菌；群落结构；多样性 中图分类号：S662.5 文献标识码：A 文章编号：1000-5684(2011) CNKI:22-1100/S1508.004 DOI: /kcms/detail/22.1100.S1508.004.html 网络出版地址： Analysis of the Bacterial Community Structures Diversity in Rhizosphere of Cerasus sachalinensis Kom. 1 1 2 1 1 1 QIN Si-jun , LÜ De-guo , LI Zhi-xia , LIU Ling-zhi , LIU Guo-cheng , MA Huai-yu 1. College of Horticulture, Shenyang Agricultural University, Shenyang 11016, China; 2. Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, China Abstract ：The bacterial community structures of Cerasus sachalinensis Kom. in topsoil and rhizospheric soil in wild and cultivated conditions were studied by means of PCR-DGGE (denaturing gradient gel electrophoresis), and their differences in the rhizosphere of Cerasus sachalinensis and other neighboring plant in the pristine growing conditions were also analyzed. Partial genes were amplified from soil bacterial community DNA using 16S rDNA gene primers. The diversity of PCR-amplified products was detected by DGGE. The results show that the bacterial community diversities in Ce