原发性高血压患者外周血白介素6基因表达及意义.docVIP

原发性高血压患者外周血白介素-6基因表达及意义 　　作者：张玲玲, 刘兴德 作者单位：贵阳医学院附院 心血管内科， 贵州 贵阳 550004 　　【摘要】 目的： 观察原发性高血压患者外周血白介素-6(IL-6)基因表达，初步探讨其与原发性高血压的关系及其意义。方法: 用放射免疫法测定62例原发性高血压患者和30例正常对照者外周血IL-6蛋白含量，用逆转录聚合酶链反应法检测外周血单个核细胞中IL-6 mRNA表达。结果： 与对照组比较，原发性高血压组患者外周血IL-6蛋白水平及外周血单个核细胞中IL-6 mRNA表达均显著增高(P0.01)。结论： IL-6可能参与了原发性高血压的病理生理过程，外周血单个核细胞的IL-6 mRNA表达上调可能是原发性高血压患者外周血IL-6蛋白水平升高的机制之一。 　　【关键词】 白细胞介素6; 基因表达; 高血压; 血液 　　The Expression of Interleukin-6 Gene in Peripheral Blood of Patients with Essential Hypertension and Its Significance 　　ZHANG Lingling, LIU Xingde 　　Department of cardiology, The Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou, China 　　[Abstract] Objective: To observe the expression changs of interleukin-6 (IL-6) in peripherial blood of patients with essential hypertension (EH), and to probe the relationgship between IL-6 mRNA level and EH. Methods: The concentrations of circulating IL-6 of 62 EH patients (group EH) and 30 normal people (group CN) were measured by using radioimmunoassay, and the expression of mRNA of IL-6 in mononuclear cells was measured with reverse transcription polymerase chain reaction. Results: The concentration of protein IL-6 in peripherial blood and IL-6 mRNA in mononuclear cell of group EH were significantly higher than those of group CN (P0.01). Conclusions: IL-6 might participate the patho-physiology process of EH.; Up-regulating expression of IL-6 mRNA may be one of the mechanisms increasing protein IL-6 in peripherial blood of EH patient. 　　[Key words] interleukin-6;gene expression; hypertension;blood 　　原发性高血压(essential hypertension,EH)是以体循环动脉压升高为主要表现的临床综合征，严重危害人类健康，但其发病机制及某些病理过程仍未完全阐明。近年来研究提示，炎症介质在EH发生发展的不同阶段发挥了重要作用，但目前针对炎症介质基因表达方面的检测与分析尚少见。2007年2月~12月检测62例EH患者外周血白介素-6(interleukin-6,IL-6)蛋白水平及单个核细胞IL-6 mRNA的表达，探讨外周血IL-6的变化与EH的关系。 　　1 对象与方法 　　1.1 对象及分组 　　按《中国高血压防治指南(2005年修订版)》制定的标准，选取心血管科住院和门诊新诊断或未经正规降压治疗的EH患者62例，同时选取正常的健康体检者30例作为对照组。排除继发性高血压、经冠状动脉造影确诊合并冠心病者、血栓出血性疾病、糖尿病、高脂血症、感染、严重肝肾疾病、自身免疫