厌氧微生物造就技能的目标和事理(国外英文资料).doc

厌氧微生物培养技术的目的和原理(国外英文资料) The purpose and principle of anaerobic microorganism culture technology Keywords: the focus is not on the standard gas standard solution of the standard gas standard, the standard gas standard, the standard solution of the standard gas, the standard gas standard, the standard gas standard, the standard gas, and the standard gas For simple and can be used for those who are of relatively low requirements for anaerobic culture of anaerobic bacteria, such as alkaline pyrobitumen gallic acid method, anaerobic tank training method, need meat medium method, etc. This experiment will mainly introduce these three, which are the most basic and commonly used anaerobic microorganism culture techniques. Some anaerobic microbes demanding training study, also has higher requirements for experimental apparatus, such as it is mainly used for strictly anaerobic bacteria isolation and culture of Mr Geithner technology, anaerobic incubator (glove box) method, etc. The anaerobic incubator has been popularized, and this experiment is briefly introduced. Basic coke has no subacid method Focal gallic acid and alkali solution (NaOH, Na2CO3 or NaHCO3) easily oxidized forms after alkaline gallic salt, can pass the oxidation and the formation of a black, brown pyrobitumen gallic orange to get rid of the oxygen in airtight container. The advantage of this approach is no special and expensive equipment, simple operation, suitable for any sealable container, can rapidly establish anaerobic environment, suitable for the preliminary experimental exploration research; And its disadvantage is that in the oxidation process can produce a small amount of carbon monoxide, for some anaerobic bacteria growth inhibition, at the same time, the presence of NaOH will absorb carbon dioxide from the airtight container, will have an unfavorable influence on the growth of some anaerobic bacteria. Use NaHCO3. Instead of NaOH, it can partially overcome the problem of carbon dioxide absorptio