_临床Ⅰ和Ⅱ期肺癌纵隔淋巴结微小转移灶的研究.pdf

·160 · 2005 3 27 3 　Chin J Oncol, March 2005, Vol 27, No.3 ·· ⅠⅡ 毛友生　张德超　张宏图　孙耘田　赵晓航　刘秀英　魏国联　刘芳 【】　　 。　32 Ⅰ Ⅱ181 , , Ber-Ep4 , HE , 。 　15 (46.9%) 21 (11.6%)HE , Ber-Ep4 , 6 HE 。N0、 N1、N2 19, 6,7 , Ber-Ep4 7, 2, 6 , 7 Ⅰb (N0)2 Ⅱb (N1)Ⅲa (N2)。32 、 , N2 0、18.8%46.9%。　, , Ber-Ep4 HE 。 。 【】　;　;　;　Ber-Ep4 Mediastinal lymph nodes mi cro-metastases in pati ents with clinical stage Ⅰ-Ⅱ lung cancer 　MAO You- sheng, ZHANGDe-chao, ZHANGHong-tu, SUN Yun-tian, ZHAO X iao-hang, LIU Xiu-y ing, WEI Guo-lian, LI U Fang .Depa tment of Tho acicSu ge y, Cance Institute(Hospital), Chinese Academy of Medical Sciences and Peking Union Medical Unive sity, B eij ing 100021, China Co esponding autho :MAO You-sheng, E-mail:Maoys @ 【Abstract】　Objectiv e　To in estigate micro-metastasis in mediastinal lymph nodes (mLN)of patients with clinical stage Ⅰ～ Ⅱlung cancer and its clinical significance.Methods　A total of 181 mLN from 32 lung cancer patients in clinical stage Ⅰ～ Ⅱ were collected during operation and their frozen sections at two different le els were examined immunohistochemically (IHC)with an anti-epithelial cell monoclonal antibody Ber-Ep4.Routine HE staining was done for comparison.The results were processed by Chi-square tests in SPSS 10.0 soft ware. Results　Fifteen of the 32 patients (46.9%)were found to ha e micro-metastasis in 21 of 181 mLN (11.6%) examined by immunohistochemical staining though routine histopathological examinations were negati e.Of those 15 cases, micro-metastasis was detected in 9 only by IHC and in 6 both by IHC and HE stainings.The positi e rate of micro-metastasis in N0, N1, and N2 stratified by routine pathology was 36.8% (7/19), 33.3%(2/6)and 85. 7%(6/7), respecti ely (N0 s N2, P0.05).When stratified according to clinical staging (cTNM ), pathological staging (pTNM)and pathological staging