稻瘟病菌dsRNA病毒筛选及其对寄主的影响.pdfVIP

湖南农业大学学报(自然科学版) 2017, 43(2):176–179. DOI:10.13331/ki.jhau.2017.02.012 Journal of Hunan Agricultural University (Natural Sciences) 投稿网址： 稻瘟病菌 dsRNA 病毒筛选及其对寄主的影响 李斌 1,2，陈传远 1,2，钟杰 1,2*，高必达 1,2* (1. 湖南农业大学植物保护学院，湖南 长沙 410128 ；2. 植物病虫害生物学与防控湖南省重点实验室，湖南 长沙 410128) 摘 要：应用纤维素吸附法对 60 个稻瘟病菌株进行真菌病毒筛选，发现 14 个菌株含有 dsRNA 病毒。对带毒 菌株 GD45 通过利巴韦林–高温结合方法得到衍生脱毒菌株 GD45–4，与带毒菌株相比，其菌丝更为饱满、菌丝 较直，在 PDA 平板上菌落形态显得更为蓬松，颜色偏白。推测菌株 GD45 dsRNA 病毒消除后会对其表现型产 生一定影响。 关 键 词：稻瘟病菌；dsRNA ；弱毒菌株 + 中图分类号：S435.111.4 1 文献标志码：A 文章编号：1007− 1032(2017)02−0176−04 Screening of dsRNA viruses and their impacts on Magnaporthe oryzae LI Bin1,2，CHEN Chuanyuan1,2，ZHONG Jie1,2*, GAO Bida1,2* (1.College of Plant Protection, Hunan Agricultural University, Changsha 410128, China; 2.Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Changsha 410128, China) Abstract: In this study, we screened the presence of mycoviruses from a collection of 60 Magnaporthe oryzae (M. oryzae) strains using the cellulose chromatography method and found that 14 strains harbored dsRNAs. A virus–curing derivative strain GD45–4 was obtained from the dsRNA–infecting strain GD45 by high temperature–ribavirin combined virus–curing methods. When compared to the original strain GD–45, strain GD45–4 showed a more full and straight hypha, and a whiter and fluffier colony morphology on PDA, indicating the elimination of dsRNAs affected the phenotype of strain GD45. Keywords: Magnaporthe oryzae ; dsRNA; hypovirulent strain 真菌病毒广泛存在于植物病原真菌中[1–2] 。大 显示出巨大的潜力[8–11]。 部分真菌病毒对寄主表现为潜伏侵染，但有部分真 笔者采用纤维素吸附法对 60