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内标法在仪器上应当叫做内标标准曲线法(国外英文资料)
内标法在仪器上应该叫做内标标准曲线法(国外英文资料)
Content of No. 1
The internal standard method should be called the internal standard curve method on the instrument.
Do standard curves of internal standard, the concentration of the internal standard substance is fixed (such as 1 PPM), and to the concentration of the analyte is a gradient (such as: 0.1 parts per million, 1 PPM, 10 PPM), so that shi analyte response values (peak area) and internal standard substance of response values than also should be a gradient (because of internal standard peak area are theoretically the same), the ratio is known as the RF. Using the RF (rather than the peak area of the analysis) the standard curve of the analysis concentration is called the internal standard curve.
Light has a standard curves of internal standard is not referred to as internal standard method, because this curve with the ordinary actually theres not much difference in standard curve (is only a numerical ordinate transformation). Only when the standard curves of internal standard combined with pretreatment process can be called a complete internal standard method (internal standard, of course, there are other purposes), then the benefits of the internal standard method reflected: 1) without the capacity, can reduce the labor intensity. 2) since it doesnt have to be fixed, the recovery rate is not too strict, as long as its reducible.
Specific operation:
1 build the internal standard curve.
The internal standard is matched to the same concentration as the internal standard in the standard curve (e.g. 1ppm).
The extracted solvent and extract of the inner subject are analyzed.
The extraction solvent is recovered and purified and purified.
The integral calculates the ratio of the analysis to the internal standard, and then the standard curve of the internal standard, and the concentration of the analytes. Internal standard (to note here is that this time is not nominal initial concentration of 1 PPMB, may be greater than or less than 1 P
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