白介素18(国外英文资料).docVIP

白介素18(国外英文资料) Interleukin - 18 (il-18) Peak biotechnology 20107040018 IL - 18 formerly known as IFN - gamma induced factor, and human liver cells from mice in the cloning of a new kind of cytokines, 1996 officially named IL - 18. The rat IL - 18 precursor protein is composed of 192 amino acids, relative molecular mass of 2.4 * 104. The N end has a special 35 amino acid precursor sequence, lacking N - glycosylation site and hydrophobic signal peptide site. Natural proteins consist of 157 amino acids, which are 1.8 by 104. The human il-18 genes encode 193 amino acid precursor proteins, and the rats il-18 has 65 percent homogeneity. The SDS - PAGE shows the human il-18 relative molecular mass is 18.3 * 103. The human il-18 is produced mainly by mononuclear, macrophages, and the mices il-18 is mainly produced by dead cells and activated macrophages. 1, the source of the IL - 18 The mRNA of il-18 can be detected in multiple organs, tissues and cells, such as thymus, liver, spleen, kidney, pancreas, dead cell and activated macrophages. Untreated mice with epidermal cells expressed constant low levels of il-18mrna. The experimental results showed that the expression of il-18mrna in epidermal cells was significantly raised in the early stage of stimulation after the stimulation of the contact allergen, and the peak was reached after the stimulation of 4-6h. Further experiments showed that keratin formation cells are the main source of il-18 in the epidermis, [3]. The osteoblast matrix cells express the il-18. In the sample does not support osteoclast multinucleated cells (OCL) formation of stromal cell line cells mRNA expression of IL - 18 quantity is higher, in support of stromal cell line cells formed by OCL expression is low [7]. Both the treatment and acute cold stress can be used to express the rats adrenal expression il-18mrna. In-situ hybridization shows that the mRNA of il-18 is mainly concentrated in the adrenal cortex, especially in the network and girdle of gluc