27 649-651 转613 食品安全及检测 3+18 高效液相色谱法测定口香糖中维生素C.pdfVIP

现代食品科技 Modern Food Science and Technology 2010, Vol.26, No.6 高效液相色谱法测定口香糖中维生素C 1,3 1,3 2,3 1,3 周芳梅 ，冯志强 ，文 ，蔡双福 （1.广东省食品质量监督检验站，广东广州510308 ）（2.广东省食品工业研究所，广东广州 510308 ） （3.广东省食品工业公共实验室，广东广州510308 ） 摘要：建立了高效液相色谱法快速测定口香糖中维生素C含量的方法。样品用0.15%草酸溶液在高温高压提取，采用高效 液相色谱法，以0.15%草酸溶液作为流动相，经AQ-C18 色谱柱分离，采用二极管阵列检测器，在246 nm 波长下测定维生素C的峰面积，并与标准系列比较，以保留时间和特性紫外光谱图定性，峰面积定量。本方法的线性范围2~ 100 μg/mL，检出限为2 g/g ，样品加标回收率在95.4~103.6% ，相对标准偏差为1.2%。本方法操作简单、灵敏度高、准确、快速，适用于口香糖中维 生素C的测定。 关键词：高效液相色谱法；维生素C；口香糖；光谱图 文章篇号：1673-9078(2010)6-649-651 Determination of Vitamin C Content in Gum by High Performance Liquid Chromatography ZHOU Fang-mei1,3, FENG Zhi-qiang1,3, WEN Can2,3, CAI Shuang-fu1,3 (1.Guangdong Food Quality Supervision and Inspection Station, Guangzhou 510308, China) (2.Guangdong Food Industry Institute, Guangzhou 510308, China) (3.Guangdong Provincial Food Industry Public Laboratory, Guangzhou 510308, China) Abstract: A rapid method for the determination of vitamin C in gum was developed by high performance liquid chromatography. Vitamin C in samples was extracted by 0.15% oxalic acid solvent at high temperature and pressure and then chromatographically separated on a C18 column using 0.15% oxalic acid aqueous solution as mobile phase and a diode-array detector at 246 nm. The linear range of the proposed method was 2~100 μg/mL. The detection limit of vitamin C was 2 μg/g. The recoveries of the samples were 95.4~103.6% with the relative standard deviation of 1.2%. The method was simple, sensitive and accurate, which can be applied to the rapid determination of vitamin C in gum. Key words: HPLC: vitamin C; gum; spectrogram 649 口香糖又