超声辐射力结合靶向超声微泡评价小鼠肾缺血再灌注损伤.PDFVIP

·402 · doi 10.3969/j.issn. 1673-4254.2017.03.22 J South Med Univ, 2017, 37(3): 402-406 基础研究 超声辐射力结合靶向超声微泡评价小鼠肾缺血再灌注损伤超声辐射力结合靶向超声微泡评价小鼠肾缺血再灌注损伤 王宝平，罗利红，吴凤林 南方医科大学南方医院超声诊断科，广东 广州 510515 摘要：目的探讨超声辐射力促进靶向微泡粘附提高肾缺血再灌注（IR）损伤检测的敏感性及可行性。方法 制作小鼠肾缺血再 灌注模型及正常对照组，并根据再灌注后1、3、6、12、24 h 分别分为IRlh、IR3 h、IR6 h、IR 12 h、IR24 h 组。各组再根据有无超声辐 射力作用随机分为单击靶向微泡（MBICAM ）组和靶向微泡+超声辐射力组（MBICAM+USRF ）组。结果 MBICAM 组和MBICAM+USRF 组在对照 组肾脏均未见明显显影增强，肾脏声强度（VI）值无显著差异（6.47±0.42 对6.45±0.62 ，P=0.923）。肾脏IR 损伤不同时间窗下均 可见显影增强，并且随着时间的推移，显影强度逐渐增高。MBICAM+USRF 组缺血再灌注各时间窗肾脏VI 值较MBICAM 组显著增 大，两者之间均存在显著性差异（P均=0.000）。IR 12 h 和IR24 hVI 值之间无显著差异（P= 1.000），其余各组之间均存在显著差异 （P0.05）。结论 应用超声辐射力联合携抗细胞间黏附分子1单抗靶向微泡可提高小鼠肾脏缺血再灌注损伤检测敏感性，可用 于早期评价微血管炎症或相关的血管内皮反应 关键词：超声辐射力；靶向微泡；缺血再灌注损伤 Evaluation of renal tissue ischemia-reperfusion injury with ultrasound radiation force and targeted microbubbles WANG baoping, LUO Lihong, WU Fenglin Department of Diagnostic Ultrasound, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China Abstract: Objective To study the sensitivity and feasibility of detecting microvascular inflammation in renal ischemia- reperfusion injury using microbubbles (MB) targeted to the intercellular adhesion molecule ICAM-1 and ultrasound radiation force (USRF). Methods Mouse models of kidney ischemia-reperfusion were randomized into 5 groups with reperfusion time of 1, 3, 6, 12, and 24 h (IRlh, IR3h, IR6h, IR12h, and IR24h group, respectively). Each group was subdivided into targeted MB group (MBICAM group) and targeted MB +USRF group (MBICAM+USRF group). Kidney enhancement and the video intensity (VI) of the kidneys were compared among the groups. Results In normal mice in either MBICAM or MBICAM + USRF group, no obvious enhancement of the kidney or significant increase in VI of the kidneys was observed (P=0.923). The kidneys were enhanced in all t