蛹虫草多糖对α-葡萄糖苷酶活性抑制研究.pdf

现代食品科技 Modern Food Science and Technology 2014, Vol.30, No.12 蛹虫草多糖对α-葡萄糖苷酶活性的抑制研究 朱振元，刘晓翠，郭蓉，赵亮，方晓娜，王振乾，张静 （食品营养与安全教育部重点实验室，天津科技大学食品工程与生物技术学院，天津 300457） 摘要：本文以蛹虫草子实体为原料，通过建立 a-葡萄糖苷酶抑制剂体外验证模型，以麦芽糖作为反应底物，阿卡波糖作为阳性 对照，利用体外酶促反应方法，对通过水提、脱色、醇沉、除蛋白及色谱柱纯化得到的不同纯度的蛹虫草多糖的 α- 葡萄糖苷酶抑制 活性进行了验证。结果表明，通过逐步的酶促反应实验，确定蛹虫草中对α-葡萄糖苷酶活性具有较显著抑制作用的功能成分为多糖， 并且随着多糖纯度的不断提高其抑制率随之提高。通过对经色谱层析柱纯化后蛹虫草多糖进一步的酶促反应实验研究，表明蛹虫草多 糖对 α-葡萄糖苷酶的抑制率随多糖浓度的提高而提高，说明蛹虫草多糖的抑制效应存在一定的剂量依赖性，纯化后的多糖对 α-葡萄 糖苷酶的半数抑制浓度（IC50）为4.22 mg/mL，呈现出良好的降血糖功能，为今后将蛹虫草开发为降血糖药物及保健品提供了一定的 理论依据。 关键词：蛹虫草；多糖；α-葡萄糖苷酶；抑制 文章篇号：1673-9078(2014)12-55-60 DOI: 10.13982/j.mfst.1673-9078.2014.12.0 10 Inhibitory of α-Glucosidase by Polysaccharides from Cordyceps militaris ZHU Zhen-yuan, LIU Xiao-cui, GUO Rong, ZHAO Liang, FANG Xiao-na, WANG Zhen-qian, ZHANG Jing (Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China) Abstract: In this study, the fruiting body of Cordyceps militaris was used to validate its α-glucosidase inhibitory activity in vitro. Using maltose as the reaction substrate and acarbose as a positive control, in vitro enzymatic reactions were carried out to validate the α-glucosidase inhibitory activity of polysaccharides (with different purities) from Cordyceps militaris, which were purified by water extraction, decolorization, alcohol precipitation, deproteinization, and column chromatography. The results of stepwise enzymatic reaction showed that th e functional component with α-glucosidase inhibitory activity in Cordyceps militaris was polysaccharide, and the inhibitory rate gradually increased with increasing purity of the polysaccharide. The results of further enzymatic reactions using Cordy