经颈静脉移植神经干细胞治疗脑性瘫痪.doc

经颈静脉移植神经干细胞治疗脑性瘫痪 娄元俊1，刘 阳2，单海军1，曹彩红1，介小素1(1河南中医学院第二附属医院，河南省郑州市 450000；2河南中医学院研究生处，河南省郑州市 450000) 引用本文：娄元俊，刘阳，单海军，曹彩红，介小素. 经颈静脉移植神经干细胞治疗脑性瘫痪[J].中国组织工程研究，2016，20(14):2092-2097. DOI: 10.3969/j.issn.2095-4344.2016.14.017 ORCID: 0000-0001-5791-0214(娄元俊) 文章快速阅读： 文题释义： 抬高身体摇摆实验：用于测量运动不对称，测量时首先用手提起大鼠的尾根部，大鼠头部悬垂距平面5 cm左右，这时大鼠头部会向左侧或右侧旋转，向单侧旋转的角度大于10°时为计数标准，记录旋转的方向和角度，一次实验后让大鼠休息1 min，再进行下一次实验，重复实验20次，记录总的次数和方向。 尼氏体：为嗜碱性物质，又称嗜染质，光镜下呈斑块状或细粒状散在均匀分布。在一些大型的运动神经元，尼氏体大而多，宛如虎皮花纹，又称虎斑。电镜下，尼氏体由大量平行排列的粗面内质网和其间的游离核糖体组成。尼氏体是神经元胞体细胞质的特征性结构之一，神经元×105个)，对照组和模 Neural stem cell transplantation for treatment of cerebral palsy Lou Yuan-jun1, Liu Yang2, Shan Hai-jun1, Cao Cai-hong1, Jie Xiao-su1 (1Second Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450000, Henan Province, China; 2Graduate Office, Henan University of Chinese Medicine, Zhengzhou 450000, Henan Province, China) Abstract BACKGROUND: Transplanted neural stem cells can survive, proliferate and differentiate into neurons and/or glial cells in the host, thereby promoting partial function recovery in the host. OBJECTIVE: To study the therapeutic effects of neural stem cell transplantation on cerebral palsy rats. METHODS: Twenty-four rats were randomly divided into three groups: control group, model group and transplantation group. Animal models of cerebral palsy were made in the latter two groups. One week after modeling, rats in the transplantation group were injected 1 mL stem cell suspension (1×105) via the jugular vein, and rats in the control and model group were given the same volume of normal saline. Toe distance, step length and elevated body swing test in rats were detected, and histopathological changes in the rat brain were observed 3 weeks after transplantation. RESULTS AND CONCLUSION: In the model group, the toe distance and step length of the front left palm were significantly lower than those of the front right palm (P 0.05). Compared with the model group, the toe distance and step