利用Berthelot颜色法测定发酵液中铵离子的干扰排除.pdf

( ) Vol. 32 No. 3 282 Journal of East China U niversity of Science and Technology ( Natural Science Edition) 200 -03 : 100 -3080( 200 ) 03-0282-03 Berthelot 马　悦, 　朱贻华, 　赵东玲, 　张元兴 ( 华东理工大学生物反应器工程国家重点实验室, 上海200237) : 利用Berthelot 反应测定微生物发酵中铵离子浓度时, 培养液中的亚硫酸钠严重干扰 了测定结果。预先加入适量的次氯酸钠溶液处理样品能有效地排除这一干扰。对次氯酸钠的加入 量、处理时间和待测样品的 进行优化, 结果表明: 当次氯酸钠与亚硫酸钠的摩尔比为2 ∶1、处理 pH 时间 1 、样品处理前调 9. 0 时, 测得发酵液中铵离子浓度, 经离子色谱法对照测试验证. min pH 最大相对误差为2. 3%, 达到了理想的效果。 : Berthelot 颜色反应; 亚硫酸钠; 次氯酸钠; 铵离子测定 : O 57. 32 : A Interference Removal in Ammonium Measurement of Fermentation Broth by Berthelot Color Reaction , - , - , - MA Yue ZH U Yi hua ZH A O Dong l ing ZH A N G Yuan x ing (State K ey L abor atory of Bioreactor Eng ineering , Ea t China Univ er ity of Science and T echnology , Shanghai 200237, China) : Abstract Sodium sulfite seriously interfered ammonium measurement in fermentation broth by Berth- elot color reaction method. Pretreatment of samples by adding proper amount of sodium hypochloride w as found effective to remove the interference. The adding amount of sodium hypochloride, pretreatment time . and sample pH were investigated It was confirmed by ion chromatography analysis that the ammonium concentration of the fermentation broth sample containing sodium sulfite including some microorganism, cells, could be determined satisfactorily by the Berthelot color reaction method modified. Key words: Berthelot color reaction; sodium sulfite; sodium hypochlorite; ammonium measurement