Matlab Digital Image Processing For Microscopy Screening显微镜筛选MATLAB数字图像处理.pptxVIP

Matlab Digital Image Processing For Microscopy Screening Javier Velasquez Pratik Dhuru Meet Vaghani BMES 546 Project Motivation Available Technologies: Confocal Microscopy Digital Imaging High Throughput Processing Potential Applications: Cell Culture Protein Expression Heterologous Protein Expression GPCR expression in Yeast/E. coli Why? Issues presented for candidate screening Protein Expression Quantification Whole Cell Fluorescence Gel Quantification How Do We Approach the Problem? GUI Demo GUI Demo GUI Demo GUI Demo Edge Detection and Counting Step 1:Read the matrix and store in matrix form Function: imread() Step 2: Convert to RGB color image to grayscale image Function: rgb2gray() Step 3:Use Sobel Operator for edge detection Function:edge() Step 5: Count no of cells Function:strel(), imerode,imfill,bwlabel Counting continued Step 6: Superimpose the edge detected image and original image to get color image Step 7: Save the new image Function: imwrite() Whole Cell Fluorescence Levels Call function: quantify(C:\Users\Javier\Pictures\Cells, [1:4], .tif) Future Work: Create Mask from image obtained from edge detection Algorithm and filter cell fluorescence from background. Cell count and detection of complicated images . Questions? Comments? Gripes?