PCR實驗室操作規範.pptxVIP

PCR实验室操作规范 及污染防治 浩源研发部 Len Chen(陈坤良) 为何要有良好的操作规范和污染防治?? 1. Infection disease: HBV , HCV, HIV 2. DNase , RNase,Contamination 3. Sample ,Product Contamination No known test method can offer complete assurance that products derived from human sources or inactivated microorganisms will not transmit infection. Therefore, all human sourced materials should be considered potentially infectious Avoid introduction of ribonucleases (RNases), nucleases(DNase) into samples during and after the extraction procedure Amplification technologies such as PCR are sensitive to accidental introduction of product from previous amplification reactions or sample. Incorrect results could occur if either the clinical specimen or the RealTime reagents used in the amplification step . Viral Particle HBV: envelope, partially double-stranded DNA virus HCV: envelope, single-stranded RNA virus HIV: envelope, single-stranded RNA virus DNase (Deoxyribonuclease):脱氧核糖核酸酶 : A deoxyribonuclease (DNase, for short) is any enzyme that catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA backbone. Phosphodiester bond RNase (Ribonuclease):核糖核酸酶 : A deoxyribonuclease (DNase, for short) is any enzyme that catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA backbone. Phosphodiester bond 1. Infection disease: HBV , HCV, HIV 2. DNase , RNase,Contamination 3. Sample ,Product Contamination No known test method can offer complete assurance that products derived from human sources or inactivated microorganisms will not transmit infection. Therefore, all human sourced materials should be considered potentially infectious Avoid introduction of ribonucleases (RNases), nucleases(DNase) into samples during and after the extraction procedure Amplification technologies such as PCR are sensitive to accidental introduction of product from previous amplification reactions or sample. Incorrect results could occur if either the clinical specimen or the RealTime reagen