陕西韩城严重发生的小麦矮缩病病原鉴定与原因分析.pdfVIP

才施和饼、); 第 34 带第 2 期(2008) 研究报费; PLANT PROTECTION Vo 1. 34 No.2 (2008) • 17 • 研究报告 Research Reports 陕西特城严重发生的小麦矮缩病病原 鉴定与原因分析 王江飞l ， 柳树宾2 ， 关蓓蕾1 ， 谢家建1 ， 王锡锋1 铃 (1.中国农业科学院植物保护研究所，极物病虫寄生物学国家囊点实验寞，北京 100094; 2. 陕西省将城市植保植检站， 715400) 摘要 2007 年在陕街串串城发现卢种新的小麦病毒病害，症状表现为严重矮缩、黄化、条斑和分集增多等，发病面积 2 约 0.07 万 hm ，病回减产达 50% ，严寞地块甚至绝收。本研究通过对采集自我国陕西韩城的 7 个样品进行 PCR 鉴 定、会基因组序列测定及比较，证实陕西韩城样品确是小麦矮缩病毒(WDV)侵染所致，并对发病原因及其流行趋势 进行了分析。这是小麦矮缩病在我国麦田大发生的首次报道. 关键词 小麦矮缩病毒(WDV); 分子鉴定z 发生 中团分类号 S 435. 121. 49 Identification and analyses of the pathogen causing the Wheat dwarf virus in Hancheng of Shaanxi Province , China 2 1 1 1 Wang JiangfeP , Liu Shubin , Wu Beilei • Xie Jiajian , Wang Xifeng (1. State Key Laboratory fo俨 Biology of Plant Díseases and Insect Pests , Institute of Plant Protection , CAAS , Beijing 100094 , Chína; 2. Hancheng Plant Protection Station , Shaanxi 715400 , Chína) Abstract A new disease wωfound on wheat in Hancheng of Shaanxi Province. The symptoms of the disease were dwarfing , mottling , yellowing and reduced heading. It was identified as Wheat dwa俨fvi俨us (WDV) by PC民 The complete genomic sequences of 7 isolates of WDV collected from Hancheng were determined. The genomic organiza瞒 tion of these isolate唱 was comparable to the other WDV isolates , and the nucleotide sequences of their whole genomes showed 96.3%-99.6% similarity. The reasons for WDV occurrence in Hancheng