Caco-2细胞单层模型的建立及其评估.pdf

药物与临床 Drugs and Clinic Caco-2细胞单层模型的建立及其评估 1,2 2 孙旭 ，罗余萍 1. 中国食品药品检定研究院国家药物安全评价监测中心、药物非临床安全评价研究北京市重点实验室，北京 100176 ；2. 南昌 大学医学院 临床药理研究所，江西南昌 330006 [摘要] 目的：建立完整的Caco-2细胞单层模型，以利于药物转运的研究。 方法：通过测量细胞生长过程中跨膜电阻(TEER) 值和碱性磷酸酶活性，评估Caco-2细胞单层模型是否建立成功。结果：细胞接种到Transwell板后的第7天，TEER值就已达到 2 2 209.75 Ω·cm ，第10天后，几乎成线性上升，直到第22天到达一个稳定值627 Ω·cm ；细胞生长的第3天，磷酸酶活性非常低， 第15天时磷酸酶活性比较高。到了第22天时，磷酸酶活性进一步增加，AP/BL 为第15天时的2倍。结论：建立的Caco-2细胞单 层模型致密且完整，检测细胞生长过程中TEER值和碱性磷酸酶活性，可用于评估Caco-2细胞单层模型建立成功。 [关键词] Caco-2细胞单层模型；跨膜电阻(TEER)值；碱性磷酸酶； [中图分类号] R965　　[文献标识码]　A　　[文章编号]　1672-2809(2015)14-0033-04 Establishment of Caco-2 cell monolayer model SUN Xu1,2, LUO Yu-ping2 1 Beijing Key Laboratory, National Center for Safety Evaluation of Drugs, National Institutes for Food and Drug Control, Beijing 100176; 2 Institute of clinical pharmacology, medical college of Nanchang university, Jiangxi Nanchang China, 330006 [Abstract] Objective: Building a complete Caco-2 cell monolayer model. Methods: Estimated the success of Caco -2 cell monolayer model by measured the TEER value and activity of alkaline phosphatase. Results: Day 7 after Caco- 2 c 2 ell planted on transwell plate, the TEER value was 209.75 Ω·cm . It increased almost linearly after day 10, and attained a 2 stable value of 627 Ω·cm in day 22. The activity of alkaline phosphatase was very low in day 3, but it becames much higher in day 15. AP/BL in day 22 was 2 times of day 15. Conclusion: The Caco-2 cell monolayer model was dense and complete ，and can be used to transport experiment. [Key words] Caco-2 cell monolayer model; TEER value; alkaline phosphatase aco-2细胞来源于人结肠癌细胞，是一种