高效离子色谱法测定烟草及烟草制品中麦芽酚的含量.pdfVIP

现代食品科技 Modern Food Science and Technology 2013, Vol.29, No.3 高效离子色谱法测定烟草及其制品中麦芽酚的含量 1 1 1 2 舒俊生 ，佘世科 ，陈开波 ，毛健 （1.安徽中烟工业有限公司技术中心，安徽合肥 230088 ）（2. 江南大学食品学院，江苏无锡 214122 ） 摘要：建立了使用阴离子交换分离柱，NaOH 淋洗液，电化学检测器测定烟草制品中痕量麦芽酚的离子色谱检测方法。方法基 于麦芽酚在NaOH 溶液中转化为阴离子，使用CarboPac PA1 型分离柱和NaOH 淋洗液进行分离；利用酚羟基在玻碳电极上的特异响 –2 应，使用ED 电化学检测器进行分析。在2.6010 ~26.00 mg∕ L 浓度范围内，工作曲线的线性相关系数为0.9999，检出限为2.26 μg∕ L ； 测定的整个过程可在15 min 内完成。方法用于烟草样品的分析，结果令人满意，加标回收率在97%~102%之间。 关键词：离子色谱；电化学检测；麦芽酚；烟草及烟草制品 文章篇号：1673-9078(2013)3-651-653 Determination of Maltol in Tobacco and Tobacco Products by High-performance Ion Chromatographic 1 1 1 2 SHU Jun-sheng , SHE Shi-ke , CHENG Kai-bo , MAO Jian (1.Research and Development Centre, China Tobacco Anhui Industrial Co., LTD, Hefei 230088 , China) (2.School of Food Science and Technology, Jiangnan University, Wuxi 214122 , China) Abstract: A new method was developed for the determination of trace maltol in tobacco products using anion-exchange column, sodium hydroxide eluent and electrochemical detector. The sodium hydroxide was used to promote ionization of maltol to its anionic form. The maltol can be eluted within 15 min by anion-exchange column CarboPac PA1 with elution of NaOH. Detection occurred by measuring the current generated when the maltol anions were oxidized on a glass carbon electrode of ED electrochemical detector. The presented method showed good linear relationship (r=0.9999) for maltol in the range of 2.60×10–2~26.00 mg/L, the detection limit was 2.26 μg∕L. This method has been applied to the determination of maltol