transforming growth factor-β1 OB-cadherin in porcine cardiac valve myofibroblast differentiation..pdf

The FASEB Journal • Research Communication Roles of transforming growth factor-1 and OB-cadherin in porcine cardiac valve myofibroblast differentiation Huan Wang,*,†,‡ Leslie A. Leinwand,†,‡ and Kristi S. Anseth*,‡,§,1 *Department of Chemical and Biological Engineering, †Department of Molecular, Cellular and Developmental Biology, ‡BioFrontiers Institute, and §Howard Hughes Medical Institute, University of Colorado, Boulder, Colorado, USA ABSTRACT Calcific aortic stenosis is a common dis- in myofibroblast differentiation.—Wang, H., Lein- ease, and some of its early causes are the activation and wand, L. A. and Anseth, K. S. Roles of transforming differentiation of resident fibroblasts to myofibroblasts growth factor-1 and OB-cadherin in porcine cardiac in response to transforming growth factor 1 (TGF- valve myofibroblast differentiation. FASEB J. 28, 1). The aim of this study was to understand how 4551–4562 (2014). TGF-1 and its downstream effector, OB-cadherin [cadherin 11 (CDH11)], regulate porcine myofibroblast Key Words: aortic valve fibrosis genome-wide transcription - phenotypes. Based on whole-genome microarrays, 95 smooth muscle actin and 107 genes are up- and down-regulated at both the early (8 h) and the late (24 h) time points of TGF-1 Calcific aortic stenosis (CAS) is a predominant treatment. Gene functions related to cell adhesion, cardiac valve disease in Western populations, affecting skeletal system development, and extracellular matrix 2–3% of people older than 65 yr (1). It is characterized are up-regulated by TGF-1, whereas oxidation-reduc-