神经母细胞瘤细胞系裸鼠转移模型及局部皮下荷瘤模型的建立.pdfVIP

. 258 史费1电髓提点lQ旦旦立足摄2主益篮直期 Chin J Oncol , Apri120JO ,Vo 1.32 , No .4 ·基础研究· 神经母细胞瘤细胞系裸鼠转移模型及局部 皮下荷瘤模型的建立 鹿洪亭董荷高强郝希伟宋华赵楠 {摘要l 目的 建立人神经母细胞瘸( NB) 裸鼠衔瘤模型，研究阳的使辑、转移机制以段实验 7 治疗的可能性。方法 体外培养人 NB 细胞系，取对数生长期瘤细胞，以 1 X 10 个/。例 lml 细胞悬液 接种子裸小鼠右侧前肢肋腹部皮下，观察衔瘤的生物学特性，并进行病理组织学检沓及基因芯片分 析，检测皮下衔瘸组织、转移瘤组织和患儿版发肿瘤组织的神经元特异性怖醇化酶(NSE) 表埠。结果 48 只裸鼠中，荷瘤成功 36 只，总荷铺率为75.0% 。其中 5 只裸鼠肿瘤体积很大，甚至达到裸鼠自身 体重的1/2 ，但未发生转移; 4 只裸鼠接种部位:k肿瘤生长，却发生全身转移而只裸鼠既有局部肿瘤 生伏，又发生转移。本组有 10 只裸鼠发生转移，转移寨沟 20.8% (10/48) 。结论 成功建立人 Nß 裸 鼠荷瘤棋划并橡Æ:传代，肿瘤的移植生长率和转移率均较高，是 Nß 体内研究理想的动物模型。 Nß 具有异质性，问能是 NB 转移的竟要原肉。 [主题词1 神经母细胞瘤; 裸鼠荷瘤模型; 转移瘤模糊 Establishment of subcutaneously transplanted and metastatic neuroblastoma models in nude mice W Hong-ting , DONG οωn ， GAO Qiang , HAO Xi-w白，川NG Hua , ZHAO Nan. IJepartment 01 Pediatric Surgery. AJfiliated Hospitα1 01 Medícal College , Qingdao University , Qing伽266003 ， China Co附spo阳ling author: DONG Qian , Email: dong. qω.n@sohu. ωm [ Abstract] Objective To establish a tumor-bearing nude mouse model of human neuroblastoma in order to study the mechanisms of neuroblastoma invasion and metastasis , and 10 investigate potential therapeutíc modalitìes in the experimental anima! mode!s. Methods A human neurob!astoma cell line was cultured in vitro. 1 x 107 cells undergoing exponential growth were collected in O. I ml of suspension and subcutaneously inoculaled into the right flank nexI to the forelimb În nude mice. 响le biological characteristics of the developed tumo!百 we附 observed ， and histopathological and DNA microaηay analyses were performed. The expressions of NSE În the subcutaneous tumor , metastatic tumor and the primary neuroblastoma tumor tiSSl附加m a p