HBx及其不同突变体对Hippo信号途径表达的影响.doc

HBx 及其不同突变体对Hippo信号途径表达的影响 陈琰琰1朱慧芬3杨道锋华中科技大学同济医学院附属同济医院感染科The effects of HBx and it’s mutant on Hippo signaling pathway and cell apoptosis in a human hepatic cell line 1Chen Yanyan, 3Zhu Huifen, 4Yang Daofeng, 1Liu Xiaocong, 4Deng Kelan, 3Huang Chunmei, 4Hu Xiaomeng, 2Jiang Suhua,3 Shen Guanxin（1Department of Biochemical and Molecular Biology，2Department of Immunology，Medical College of Shihezi University;Shihezi,Xinjiang Uygur Autonomous Region,832003;3Department of Immunology，epartment of infection ,Tongji Hospital;Tongji Medical College; HUST; Wuhan;Hubei Province;430030） To explore the relationship of HBx and its mutant to Hippo signaling pathway and the effects of HBx on cell cycle and apoptosis.Methods L02 cells were transfected with HBx and its mutant.After 48h of incubation, total protein was obtained and the expression levels as well as the phosphorylation status of Mst1/2 and YAP were detected by Western blot. The apoptosis of L02 cell was examined cytofluorometrically by Annexin V/PI staining. Results After transfection,the results of Western blot showed that the expression level of P-MST1/2 and YAP were up-regulated and the level of MST1/2 and P-YAP were down-regulated,In the results of flow cytometry,more apoptosis cells were observed in experimental group compared with the control group,the trend is particularly apparent for early apoptosis. Conclusion HBx could regulate the expression of YAP through Hippo signaling pathway .This mechanism may relevant to HBV related HCC,combined with the results of flow cytometry,and it suggested that HBx is a multifunctional protein that regulates the cell cycle in several ways. ［Key words］hippo YAP HCC HBV HBx apoptosis Supported by National Science and Technology subprojects of 973 Program （2007Cb2906）E-mail: guanxin_shen@ ；Jiang Suhua E-mail:jshjane@ （2007Cb2906）  越来越多的研究表明，HBx是一种多功能蛋白，可通过反式激活机制调控细胞周期和信号转导，影响细胞周期，在乙型肝炎病毒相关疾病发生起重要作用。Hippo-YAP被认为是与肿瘤发生密切相关的一条信号通路。本研究初步探寻（hepatitis B virus X protein，HBx)