健脾益气中药对脾虚大鼠骨骼肌线粒体损伤的保护作用.docVIP

健脾益气中药对脾虚大鼠骨骼肌线粒体损伤的保护作用 宋雅芳1 刘友章2 黄晓燕1 姬爱冬1 宋雅芳，黄晓燕，姬爱冬，广州中医药大学，广东省广州市 510405 刘友章，广州中医药大学第一附属医院，广东省广州市 510405 作者贡献分布：此课题由宋雅芳及刘友章设计；研究过程由宋雅芳与黄晓燕、姬爱冬操作完成；研究所用试剂及分析工具由宋雅芳提供；数据分析由宋雅芳完成；本论文写作由宋雅芳、黄晓燕完成。 广州中医药大学博士后科研基金资助 通讯作者：刘友章，510405，广东省广州市机场路12号，广州中医药大学第一附属医院.liu408@126.com 电话：020The protection of invigorating the spleen and tonifying Qi herbs on impaired mitochondria of skeletal muscle in rats with spleen asthenia Ya-Fang Song , You-Zhang Liu, Xiao-Yan Huang, Ai-Dong Ji Ya-Fang Song, Xiao-Yan Huang, Ai-Dong Ji, Guangzhou university of Chinese Medicine,Guangzhou 510405, Guangdong province, China You-Zhang Liu ,The first affiliated hospital of Guangzhou university of Chinese Medicine,Guangzhou 510405, Guangdong province, China Supported by: Post-doctor scientific research foundation of Guangzhou university of Chinese Medicine Correspondence to: You-zhang Liu,the first affiliated hospital of Guangzhou university of Chinese Medicine,Guangzhou 510405, Guangdong province, China. Liu408@126.com Abstract AIM: To observe the effects of invigorating the spleen and tonifying Qi herbs on mitochondria succinate dehydrogenase, cytochrome oxidase activity of skeletal muscle in rats with spleen asthenia. METHODS: The rats in the model group were fed with half full and perfuse with Xiaochengqi Decoction every day to build animal model of spleen asthenia.40 SD rats were randomly divided into 4 groups: normal control group, model of spleen asthenia group, sijunzi decoction group and huangqi sijunzi decoction group.General state of rats were observed,the changes of mitochondria succinate dehydrogenase, cytochrome oxidase activity of skeletal muscle were measured by biochemical method. RESULTS: Compared with normal control group, the level of SDH and cytochrome oxidase activity of skeletal muscle mitochondria in spleen asthenia group were decreased obviously（27.112±7.104 VS 44.328±5.894, 3.029±0.501 VS 4.455±0.767,both P＜0.01）；While Huangqi sijunzi decoction group and sij