利用组学技术检测转基因作物非期望效应.pdfVIP

业生物技术学报 Journal of Agricultural Biotechnology 2005,13 (6): 802~807 ·综述· 利用“组学”技术检测转基因作物非期望效应的潜在性* 欣** 黄昆仑** 朱本忠 唐茂芝 罗云波*** (中国农业大学食品科学与营养工程学院食品生物技术实验室，北京 100083) 摘要：检测由遗传修饰引起的非期望效应是进行转基因作物安全性评价的一项重要内容。以“实质等同性”概念为基础的 评价方法，重点对转基因作物和非转基因亲本中的关键性成分进行定性、定量分析和比较难以充分考虑到一些不可预见因素 的发生。“组学”技术通过对转基因作物的整个功能基因组、蛋白质组以及代谢组进行分析，将结果与非转基因亲本进行比较， 。“组学”技术是很有潜力的。综述介绍了功能基因组 蛋 完善了目前所广泛采用的以“实质等同性”概念为基础的定向方法 学、 白质组学和代谢组学所用技术的原理和方法，重点讨论了这三大“组学”技术在评价转基因作物非期望效应中的应用和局限 性，介绍了转基因食品食用安全性评价中非期望效应检测方面的最新进展。 关键词：安全评价;实质等同性;功能基因组学;蛋白质组学;代谢组学 Potentiality of “Omics”Techniques for the Detection of Unintended Effects in Genetically Modified Crops LI Xin** HUANG Kun-Lun** ZHU Ben-Zhong TANG Mao-Zhi LUO Yun-Bo *** The detection of unintended effects is a crucial step in the safety evaluation of genetically modified (GM) crops. Target- ed approach based on the principle of “Substantial Equivalence”focusing on the qualitative and quantitative analysis of key elements between GM crop and its counterpart hardly cover the occurrence of unpredictable changes. The functional genomics, proteomics and metabolomics are evolved to detect unintended effects, which might in future complement present widely be used as targeted ap- proach. The three “omics”techniques are potentially powerful, but much work has still to be done. In this review, the techniques are described, together with their current applications and limitations, introducing recent study progresses in the aspect of the detection of unintended