2008-Pikm--clone(需要两个R基因共同作用才提供抗性).pdf

Copyright 2008 by the Genetics Society of America DOI: 10.1534/genetics.108.095034 Two Adjacent Nucleotide-Binding Site–Leucine-Rich Repeat Class Genes Are Required to Confer Pikm-Specific Rice Blast Resistance ,1 † ‡ ‡ § Ikuo Ashikawa,* Nagao Hayashi, Hiroko Yamane, Hiroyuki Kanamori, Jianzhong Wu, Takashi Matsumoto,§ Kazuko Ono** and Masahiro Yano** *NARO, National Institute of Crop Science, Tsukuba, Ibaraki 305-8518, Japan, †Plant Disease Resistance Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan, ‡Institute of the Society for Techno-Innovation of Agriculture, Forestry, and Fisheries, Tsukuba, Ibaraki 305-0854, Japan, §Plant Genome Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan and **QTL Genomics Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan Manuscript received August 9, 2008 Accepted for publication October 15, 2008 ABSTRACT The rice blast resistance gene Pikm was cloned by a map-based cloning strategy. High-resolution genetic mapping and sequencing of the gene region in the Pikm-containing cultivar Tsuyuake narrowed down the candidate region to a 131-kb genomic interval. Sequence analysis predicted two adjacently arranged resistance-like genes, Pikm1-TS and Pikm2-TS, within this candidate region. These genes encoded proteins with a nucleotide-bindin