核磁共振氢谱对6群肺炎链球菌荚膜多糖结构探析.docVIP

核磁共振氢谱对6群肺炎链球菌荚膜多糖的结构分析 　　[摘要] 目的 对6群型肺炎链球菌荚膜多糖进行结构分析。 方法 应用分子生物学方法将6群肺炎链球菌分为6A型、6B型、6C型和6D型，提纯其荚膜多糖，并采用核磁共振氢谱（1H NMR）对6群肺炎链球菌荚膜多糖的结构进行分析，比较6A型、6B型、6C型和6D型多糖结构1H NMR谱的端基质子区和环质子区化学位移的差异。 结果 6A型谱图中，三个特征质子峰化学位移值分别为？啄5.62、？啄5.13和？啄5.05；6C型谱图中，三个特征质子峰化学位移值分别为？啄5.59、？啄5.13和？啄5.05；6B型谱图中，三个特征质子峰化学位移分别为？啄5.62、？啄5.17和？啄5.15；6D型谱图中，三个特征质子峰化学位移分别为？啄5.59、？啄5.17和？啄5.15。6A、6B、6C和6D型的环质子区（？啄4.40～3.30）化学位移表现出不同的位移特性。 结论 获得6A型、6B型、6C型和6D型肺炎链球菌荚膜多糖1H NMR谱图，其端基质子区和环质子区化学位移存在差异，为荚膜多糖质量控制提供基础 [关键词] 肺炎链球菌荚膜多糖；核磁共振氢谱；端基质子；环质子；化学位移 [中图分类号] R914 [文献标识码] A [文章编号] 1674-4721（2016）10（b）-0016-04 [Abstract] Objective To analyze the structures of the capsular polysaccharides from streptococcus pneumoniae serogroup 6. Methods Serotypes of streptococcus pneumonia were determinated as 6A， 6B， 6C and 6D using the molecular biological technologies， then the capsular polysaccharides were extracted and the structures were analyzed by 1H nuclear magnetic resonance （NMR）. The chemical shifts of anomeric proton regions and ring proton regions of 1H NMR spectrums were analyzed and compared among different serotypes of capsular polysaccharides. Results The chemical shifts of all characteristic protons peaks of the pneumococcal capsular polysaccharides serotype 6A were ？啄5.62， ？啄5.13 and ？啄5.05 separately； serotype 6C were ？啄5.59， ？啄5.13， and ？啄5.05； serotype 6B were ？啄5.62， ？啄5.17 and ？啄5.15； serotype 6D were ？啄5.59， ？啄5.17， and ？啄5.15. The chemical shifts of ring proton regions （？啄4.40-3.30） of 4 serotypes were different. Conclusion The 1H NMR spectrums of pneumococcal capsular polysaccharides serotype 6A， 6B， 6C and 6D were determinated， and the chemical shifts of anomeric proton regions and ring proton regions were different in each serotype. It provides data for the quality control of different capsular polysaccharide. [Key words] Pneumococcal capsular polysaccharide； 1H NMR； Anomeric proton； Ring proton； Chemical shift 肺炎链球菌（Streptococcus pneumoniae）是严重威胁人类健康的常见病原菌之一，能够引起肺炎、急性呼吸道感染、中耳炎、脑膜炎和