···Combinatorial engineering of intergenic regions.pdf

Pfleger et al. Combinatorial engineering of intergenic regions to tune expression of multiple genes in operons 1 1 1,† Brian F. Pfleger , Douglas J. Pitera , Christina D. Smolke , and Jay D. Keasling1,2,3* Note: This work is published in recent issues of Nature Biotechnology and Metabolic Engineering. All figures and tables are presented there. Pfleger, B.F., Pitera, D.J., Smolke, C.D. and Keasling, J.D., 2006. Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes. Nat Biotechnol.24 8 1027-32. /nbt/journal/v24/n8/abs/nbt1226.html Pfleger, B.F., Pitera, D.J., Newman, J.D., Martin, V.J.J., and Keasling, J.D., (In Press). Microbial Sensors for Small Molecules: Development of a Mevalonate Biosensor. Metab Eng. /science/journal 1 Department of Chemical Engineering, University of California Berkeley, CA 94720-1462 2 Department of Bioengineering, University of California Berkeley, CA 94720-1762 3 Synthetic Biology Department, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 *To whom correspondence should be addressed. Mailing address: Berkeley Center for Synthetic Biology, 717 Potter Street, Building 977, Mail code 3224, University of California, Berkeley, CA 94720-3224. USA. Phone: (510) 495-2620. Fax: (510) 495- 2630. E-mail: keasling@ †Current address: Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125 Page 1 Pfleger et al. Many applications of synthetic biology require the balanced expression of multiple