斑马鱼胚胎发育技术在毒性评价中的应用.pdfVIP

应 用 生 态 学 报 2002 年2 月 第 13 卷 第2 期 CH N E SE JOURNAL OF APPL ED ECOLOGY, Feb. 2002, 13(2)B252~ 254 * * * 朱 琳 史淑洁 ( 南开大学环境科学与工程学院, 天津 300071) = 对斑马鱼( Brachydanio rerio) 胚胎发育技术在环境科学领域的应用作一 述. 斑马鱼胚胎毒性技 术是各国际标准组织认可的标准毒性测定方法之一, 属于致畸效应检验. 该项技术成本低、易操作、灵敏度 高, 特别是具有可记录多项毒性指标的特点, 并可以此判断污染物的致毒机理. 斑马鱼胚胎发育过程受重 金属影响较大, 其中Cu 的毒性最强, Hg 次之, Cr 最弱; 有机农药中三苯基锡类( TP TA) 的毒性最强, 林丹 次之; 有机试剂中含卤素取代基和苯胺类毒性最大. 这与其它毒性测定方法的结果完全一致, 并表现出较 高的灵敏度, 特别是选用非致死性的指标. 可以初步认定这对测定复合污染物毒性并分析毒物的致畸效应 方面有很好的发展前景. 斑马鱼 胚胎发育 毒性评价 100 1- 9332(2002) 02- 0252- 03 X502 A Utilization of embryo development technique of Bra chydan io r er io to evaluateing toxicity on various chemicals. ZHU Lin and SH Shuj ie ( College of Environmental Science and Engineering, Nankai Univer sity, Tianj in 300071) .2Chin .J . A l . Ecol . , 2002, 13(2) : 252~ 254 . The application of Brachydanio rerio embr yo development technique to research on environmental science was summarized in this paper. This testing technique was approved as one of standard met hods for chemical toxicity test , esp ecially for ter atogen sis, by international or ganizations ( OECD, D N) . There are many advantages of this testing, e. g . , low cost , easy op eration , high sensitivity and multiple sensible endpoints, comp ar ing with other toxicity t esting . Also, it was u sed to judge toxic mechanism of chemicals. T he embr yo develomp ent of zebrafish can be affected obviously by heavy metal, pesticide, organic r eagent and complex chemicals. Among them, Cu and Hg appeared stronger toxicity, and Cr was w