用gfp基因检测饭豆根瘤菌在饭豆根部的定殖动态.pdfVIP

24 1 Vol. 24 No. 1 2005 2 JournalofHuazhongAgriculturalUniversity Feb. 2005, 49~ 51 gfp * * * 冯 娜 郑慧芬 何绍江 石迪萌 张 薇 (华中农业大学生命科学技术学院, 武汉 430070) Detect the Colon ization of Rh izob ium in R ice B ean by gfp Gene FengNa ZhengHuifen HeShaojiang ShiDi eng ZhangW ei (College of Lfi e Science and Technology, H uazhong Agricultural University, Wuhan 430070, China) ; ; AM F; K ey w ord s rice bean(Vigna um ellate L. ); rhizobiu ; AMF; colonization + S 154. 38 1 (Vigna um ellata L. ) CaCl 6H O 0. 1 g; Rh 4 L(Rh 2 2 , : H BO 5 g, NaMnO 5 g, dH O 1 3 3 2 4 2 , 000 L); dH O 1000 L, pH6. 8~ 7. 0; 2 , 50 g/ L , Fahraeus: CaCl 2H O 0. 1 g; 2 2 AMF, gfp MgSO 7H O 0. 12 g; KH PO 0. 1 g; NaHPO 4 2 2 4 2 4 AMF 12H O 0. 15 g; 5 g; Gibson 1 2 L (G ibson: H BO 2. 86 g; ZnSO 3 3 4 7H O 0. 22 g; MnSO 4H O 2. 03 g; NaM oO 1 2 4 2 2 4 2H O 0. 126 g; CuSO 5H O 0. 08 g dH O 1 2 4 2