一测多评法与外标法测定胆黄连中生物碱类成分含量比较..docVIP

一测多评法与外标法测定胆黄连中生物碱类成分含量比较 　　摘要：目的 建立一测多评法（QAMS）测定胆黄连中3种生物碱成分含量，并与外标法测定结果进行比较，探讨QAMS的可行性。方法 采用高效液相色谱法，以盐酸小檗碱为内参物，建立盐酸小檗碱与盐酸巴马汀、盐酸药根碱间的相对校正因子，用所得相对校正因子进行含量计算（计算值），实现一测多评。同时采用外标法测定3个成分的含量（实测值），比较计算值与实测值的差异。结果 10批胆黄连中3种生物碱成分含量的计算值与实测值无显著差异。结论 QAMS可用于胆黄连中生物碱类成分的含量测定 关键词：一测多评法；外标法；胆黄连；生物碱；相对校正因子 DOI：10.3969/j.issn.1005-5304.2016.11.019 中图分类号：R284.1 文献标识码：A 文章编号：1005-5304（2016）11-0078-03 Abstract： Objective To establish QAMS method to determine the contents of three alkaloids in bile processed Coptidis Rhizoma； To compare the results of QAMS with those from external standard method； To prove the feasibility of QAMS. Methods An HPLC method was developed. Berberine hydrochloride was selected as the internal reference substance. 2 relative correction factors （RCF） of berberine hydrochloride to palmatine hydrochloride and to jatrorrhizine hydrochloride were established. Obtained RCFs were used to conduct content calculation （calculated value） to complete QAMS method. At the same time， the contents （measured value） of the three components were also determined by external standard method. Calculated value and measured value were compared. Results The analysis results showed that there was no significant difference between the calculated values and the measured values of the three alkaloids in 10 batches of bile processed Coptidis Rhizoma. Conclusion The QAMS method can be applied in the determination of alkaloids in bile processed Coptidis Rhizoma. Key words： QAMS； external standard method； bile processed Coptidis Rhizoma； alkaloids； relative correction factors 胆黄连是黄连的炮制品之一，以猪胆汁炮制后，黄连的寒性增强，是“寒者益寒”“从制”的经典实例[1]。本试验在前期确定胆黄连炮制工艺、药效作用的基础上，采用一测多评法（QAMS）对胆黄连中盐酸药根碱、盐酸巴马汀与盐酸小檗碱含量进行研究，并将外标法和QAMS测定结果进行比较，建立胆黄连中3种生物碱类成分QAMS的含量测定方法，为胆黄连的质量评价和炮制机理研究提供依据 1 仪器与试药 Agilent1100高效液相色谱仪（chemistation system工作站），SG3300型超声波清洗器（上海冠特超声仪器有限公司），赛多利斯BT25S分析天平 黄连购自大连权健中药饮片有限公司（批产地四川），经辽宁中医药大学鉴定教研室李峰教授鉴定为毛茛科植物黄连Coptis chinensis Franch.的干燥根茎；猪胆汁购于大连猪肉市场；胆黄连饮片自制10批（黄连饮片，加6%猪胆汁水